Profiles of differential expression of miRNAs in the late stage of red blood cell preservation and their potential roles - 09/10/24

Doi : 10.1016/j.tracli.2024.09.003

Yajie Wang ^a,^{^{1
These authors have contributed equally to this work and share first authorship.}}, Yiming Ma ^a,^{^{1
These authors have contributed equally to this work and share first authorship.}}, Liping Sun ^b, Quan Rao ^c, Xiaozhou Yuan ^a, Yan Chen ^a, Xiaofei Li ^a,^⁎
^a Department of Blood Transfusion, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
^b Department of Transfusion Medicine, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China
^c Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China

^⁎Corresponding author at: Department of Blood Transfusion, Beijing Friendship Hospital, Capital Medical University, No. 95 Yong-an Road, Xi-Cheng District, Beijing 100050, China.Department of Blood TransfusionBeijing Friendship HospitalCapital Medical UniversityNo. 95 Yong-an Road, Xi-Cheng DistrictBeijing100050China

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Highlights

•	We screened the expression profiles of miRNAs in the late stage of RBC preservation.
•	We identified miRNAs that changed during different storage times as well as potential target genes.
•	We predicted the potential regulatory effects of these differential miRNAs through bioinformatics.

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Abstract

Objective

To detect the differentially expressed regulatory miRNAs in the late stage of red blood cell (RBC) preservation and predict their roles.

Methods

Suspended RBCs with different storage periods of 35 day, 42 day, and 50 day were collected for routine blood tests, RNA extraction, and preparation of small RNA sequencing libraries. The constructed libraries were sequenced and the biological functions of differential miRNAs in RBCs in the late storage were analyzed by bioinformatics.

Results

Routine indicators of RBCs in the late stage were not significantly affected by preservation time. The Pearson correlation analysis performing on RBC miRNAs with different storage days revealed that RBC miRNAs changed with the increase of storage days. RBC miRNAs from day 35 (D35), day 42 (D42) and day 50 (D50) showed significant differences (P < 0.05). Compared RBC miRNAs from D42 with these from D35, there were 690 up-regulated miRNAs and 82 down-regulated miRNAs; compared RBC miRNAs from D50 with these from D35, there were 638 up-regulated miRNAs and 123 down-regulated miRNAs; compared RBC miRNAs from D42 with these from D50, there were 271 up-regulated miRNAs and 515 down-regulated miRNAs. GO enrichment analysis of target genes of differential miRNAs were mainly involved in cell metabolism, biosynthesis, protein modification, gene expression and transcriptional regulation of biological processes. KEGG pathway enrichment analysis of miRNA target genes showed that differential miRNA target genes were closely related to pathways in cancer.