Epidemiology and molecular characterization of Mycobacterium tuberculosis including a drug-resistant strain associated with mortality of Asian elephants in Nepal 2019–2022 - 05/09/24

Doi : 10.1016/j.tube.2024.102550

Arjun Pandit ^a,^{^{1
A.P. and J.T. contributed equally to this work.}} , Jeewan Thapa ^b,^{^{1
A.P. and J.T. contributed equally to this work.}} , Amir Sadaula ^c , Yasuhiko Suzuki ^b , Chie Nakajima ^b , Susan K. Mikota ^d , Naresh Subedi ^c , Bijaya Kumar Shrestha ^e , Michito Shimozuru ^a , Bhawana Shrestha ^f , Bijendra Raya ^f , Sanjay Chaudhary ^f , Sarad Paudel ^g , Toshio Tsubota ^a,^⁎
^a Laboratory of Wildlife Biology and Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, 060-0818, Sapporo, Japan
^b Hokkaido University International Institute for Zoonosis Control and Institute for Vaccine Research and Development, Kita 20 Nishi 10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan
^c National Trust for Nature Conservation, Kathmandu, Nepal
^d Elephant Care International, Hohenwald, TN, USA
^e Department of National Parks and Wildlife Conservation, Kathmandu, Nepal
^f German Nepal Tuberculosis Project, Kathmandu, Nepal
^g College of Veterinary Medicine, Long Island University, New York, USA

^⁎Corresponding author.

Abstract

Tuberculosis (TB) is an emerging threat to the survival of elephants in Nepal. We investigated the lung tissue samples from nine elephants that died from 2019 to 2022 in Nepal using culture, conventional PCR, and loop-mediated isothermal amplification (LAMP) and then performed genotyping of five PCR-positive isolates to understand the possible transmission dynamics of Mycobacterium tuberculosis (Mtb). Results showed that two-thirds (6/9) of elephants were confirmed to be infected from Mtb by LAMP, 5/9 by PCR, and 4/9 by culture. Genotyping of Mtb isolates showed that elephants were infected with the Indo-Oceanic and Beijing lineages including an isoniazid-resistant Beijing lineage. MIRU-VNTR-based phylogeny, gyrA, and katG sequencing showed the possibility of ongoing transmission of Indo-Oceanic lineages and likely transmission of the drug-resistant Beijing lineage from human to elephant. Implementation of comprehensive surveillance and preventive measures are urgently needed to address this zoonotic disease and protect elephants from TB in Nepal.

Le texte complet de cet article est disponible en PDF.

Keywords : Elephant, Tuberculosis, Nepal, Genotyping, Transmission dynamics

Plan

Export

Vol 148

Article 102550- septembre 2024 Retour au numéro

Article précédent

Host urinary biomarkers in HIV positive and HIV negative patients with tubercular uveitis and other uveitic diseases
Dian P. van der Westhuizen, Candice I. Snyders, Martin Kidd, Gerhard Walzl, Novel N. Chegou, Derrick P. Smit

| Article suivant

Effect of Metformin on systemic chemokine responses during anti-tuberculosis chemotherapy
Nathella Pavan Kumar, Chandrasekaran Padmapriyadarsini, Arul Nancy, M. Tamizhselvan, Anant Mohan, Devarajulu Reddy, N. Poorana Ganga Devi, Prabakaran Rathinam, Bharathi Jeyadeepa, R.K. Shandil, Randeep Guleria, Manjula Singh, Subash Babu

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

Déjà abonné à cette revue ?

connectez-vous ou créez un compte

Epidemiology and molecular characterization of Mycobacterium tuberculosis including a drug-resistant strain associated with mortality of Asian elephants in Nepal 2019–2022 - 05/09/24

Abstract

Plan

Export citations

Fichier

Contenu

Accès rapides

Mon compte

Aide & support

Plateformes Elsevier Masson

Déclaration CNIL