Differential diagnostic value of simultaneous detection of CD69 and HLA-DR on host T and NK cells in QFT-TB assay for identifying active tuberculosis - 05/09/24
, Fujie Zhang c, 1 , Hanlu Shi d , Zhongliang Zhu a , Yu Zhou a, ⁎ , Yonglie Zhou a, ⁎ Abstract |
Background |
Interferon-gamma release assay (IGRA) for tuberculosis (TB) remains limited in its ability to discriminate between active TB (ATB) and latent TB infection (LTBI). Activation markers on host T and NK cells are currently considered to be promising markers in the diagnosis of ATB.
Methods |
This prospective observational study enrolled 213 participants and the participants were divided into ATB, LTBI, other lung-related diseases (ORD), and health control (HC) groups. CD69 and HLA-DR on T and NK cells were detected in QFT-TB assay, and a composite scoring system (TB-Flow) was created for the diagnosis of ATB.
Results |
The expression of activation markers (CD69 and HLA-DR) were significantly increased in ATB. HLA-DR on NK cells, CD69 on T cells, and QFT-TB in the differential diagnosis of ATB and HC were all of good diagnostic value (AUC>0.90). In addition, the TB-Flow greatly improved the efficiency of differential diagnosis between ATB and LTBI (AUC=0.90, 95%CI: 0.84–0.96), with sensitivity and specificity of 79.17 % (95%CI: 64.60%–89.04 %) and 88.68 % (95%CI: 76.28%–95.31 %).
Conclusions |
CD69 and HLA-DR on host T and NK cells are promising markers in distinguishing different TB infection status. Our blood-based TB-Flow scoring system can distinguish ATB from LTBI with good diagnostic efficacy.
Le texte complet de cet article est disponible en PDF.Keywords : CD69, HLA-DR, ATB, LTBI, TB-Flow
Plan
Vol 148
Article 102537- septembre 2024 Retour au numéro
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