Protective effects of forsythoside A against severe acute pancreatitis- induced brain injury in mice - 21/08/24
Abstract |
Objectives |
This study aimed to evaluate the therapeutic effects of forsythoside A (FA) on brain injury induced by severe acute pancreatitis (SAP) using a murine model.
Methods |
Mice were induced with 3.5 % sodium taurocholate to model SAP-induced brain injury (SAP-IBI) and were randomly assigned to four distinct treatment regimens: the SAP-IBI model group (SAP-IBI), low-dose FA treatment group (FA L+SI), middle-dose FA treatment group (FA M+SI), and high-dose FA treatment group (FA H+SI). A sham-operation group (SO) served as a negative control. Serum levels of interleukin-1β (IL-1β) and IL-18 were quantified via ELISA, and serum amylase levels were assessed using optical turbidimetry. mRNA expression levels of AIM2, ASC, Caspase-1, and GAPDH in hippocampal brain tissue were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Protein levels of NLRP3, GSDMD, IL-1β, and IL-18 in hippocampal brain tissue were evaluated using Western blotting. Neurological function in surviving mice was assessed through modified neurological severity scores (mNSS). Transmission electron microscopy (TEM) provided ultrastructural analysis of the hippocampus. Additionally, water content and pathological changes in hippocampal brain tissue were examined 24 hours post-operation, along with other relevant indicators.
Results |
At 24 hours post-operation, the FA H+SI group exhibited significantly reduced levels of serum amylase, IL-1β, and IL-18, along with decreased expression of AIM2, ASC, and Caspase-1 mRNA. Furthermore, NLRP3 protein levels, water content, pancreas and hippocampal brain pathological scores, and mNSS were significantly lower compared to the SAP-IBI group (P<0.01).
Conclusions |
FA demonstrates protective effects against SAP-IBI in mice, suggesting potential therapeutic benefits.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Pyroptosis is crucial in severe acute pancreatitis-induced brain injury. |
• | FA significantly mitigates inflammation and brain damage. |
• | FA effectively inhibits the AIM2 inflammasome, reducing key pyroptosis markers. |
• | Strong correlation exists between biomarkers and the severity of brain pathology. |
• | FA demonstrates potential for therapeutic application in various neuroinflammatory diseases. |
Keywords : Forsythoside A, SAP-IBI, AIM2, Pyroptosis
Plan
☆ | This study was self-funded. |
Vol 178
Article 117301- septembre 2024 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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