Rechercher
image du produit
American Journal of Infection Control

S'abonner

Genetic relatedness, antibiotic resistance, and virulence of Klebsiella pneumoniae isolated from health care and food sources in Wuhan, China - 15/08/24

Doi : 10.1016/j.ajic.2024.05.011 
Feng Tang, PhD a, 1, Zhi Chen, MD b, 1, Hanjue Zhu, BD c, 1, Lei Xi, MD a, ⁎ , Changzhen Li, BD a, ⁎ , Wanjun Luo, MD d, ⁎
a Department of Laboratory Medicine, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China 
b Microbiological Laboratory, Wuhan Center for Disease Control and Prevention, Wuhan, China 
c Plastic Surgery, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China 
d Hospital-Acquired Infection Control Department, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China 

⁎Address correspondence to Lei Xi and Changzhen Li, Department of Laboratory Medicine, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430016, China or Wanjun Luo, Hospital-Acquired Infection Control Department, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430016, China.Department of Laboratory Medicine, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430016, China or Wanjun Luo, Hospital-Acquired Infection Control Department, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & TechnologyWuhan430016China

Résumé

Background

To investigate genetic relatedness and antibiotic resistance of Klebsiella pneumoniae from retail meat samples, clinical source samples, and hospital environmental samples in Wuhan, China.

Methods

Hypermucoviscosity and biofilm formation of K. pneumoniae were assessed by string test and crystal violet staining. MICs of 18 antimicrobials were determined by broth microdilution. PCR detected 14 antibiotic resistance genes. Genetic relatedness and clonal dissemination were analyzed by PFGE.

Results

Among 5,730 samples, 46 were tested positive for K pneumoniae, with higher rates observed in meat (23.4%) than in clinical samples (0.6%) and hospital environmental samples (8.0%). Meat-derived isolates showed high resistance to tetracycline (36.4%, 4/11), sulfonamide (27.3%, 3/11), and gentamicin (27.3%, 3/11), whereas clinical isolates exhibited significant resistance to ampicillin-sulbactam (32.3%, 10/31). Multidrug resistance was observed in 17.4% (8/46) of the isolates, particularly in hospital environmental samples (3/4). Biofilm production was observed in 88.1% (37/42) of K pneumoniae. Pulsed-field gel electrophoresis analysis revealed patient-to-patient K pneumoniae transmission, transmission between patients and hospital environment, as well as cross-contamination between markets.

Conclusions

The findings underscore the importance of comprehensive surveillance, infection control, and judicious antibiotic use in mitigating the impact of K pneumoniae on public health, especially in the food chain and health care settings.

Le texte complet de cet article est disponible en PDF.

Highlights

•
Detection of Klebsiella pneumoniae in pediatric patients, clinical settings, and foodstuff.
•
Virulence phenotypes and biofilm formation of K pneumoniae from different sources.
•
Antibiotic resistance and resistance genes of K pneumoniae from different sources.
•
Homology analysis indicates its transmission between different sources.

Le texte complet de cet article est disponible en PDF.

Key Words : PFGE, Resistance profile, Foodborne infection


Plan


 Funding/support: This study was supported by the National Natural Science Foundation of China (Grant No. 82072351), Wuhan Health Bureau of China for the clinical research project (WX20D30, WX20C10), and Wuhan Municipal Health Youth Talent Training Program (2021).
 Conflicts of interest: All authors declared no conflict of interest existed in this work. All authors are aware of and agree to the content of the paper and their being listed as a co-author of the paper.
 Consent for publication: All authors declared no conflict of interest existed in this work. All authors are aware of and agree to the content of the paper and their being listed as a coauthor of the paper.


© 2024  Association for Professionals in Infection Control and Epidemiology, Inc.. Publié par Elsevier Masson SAS. Tous droits réservés.
Ajouter à ma bibliothèque Retirer de ma bibliothèque Imprimer
Export

    Export citations

  • Fichier

    •

  • Contenu

    •

Vol 52 - N° 9

P. 1043-1051 - septembre 2024 Retour au numéro
Article précédent Article précédent
  • Carbapenem-resistant Acinetobacter baumannii complex in the United States—An epidemiological and molecular description of isolates collected through the Emerging Infections Program, 2019
  • Sandra N. Bulens, Davina Campbell, Susannah L. McKay, Nicholas Vlachos, Alex Burgin, Mark Burroughs, Jasmine Padila, Julian E. Grass, Jesse T. Jacob, Gillian Smith, Daniel B. Muleta, Meghan Maloney, Bobbie Macierowski, Lucy E. Wilson, Elisabeth Vaeth, Ruth Lynfield, Sean O’Malley, Paula M. Snippes Vagnone, Jennifer Dale, Sarah J. Janelle, Christopher A. Czaja, Helen Johnson, Erin C. Phipps, Kristina G. Flores, Ghinwa Dumyati, Rebecca Tsay, Zintars G. Beldavs, P. Maureen Cassidy, Amanda Hall, Maroya S. Walters, Alice Y. Guh, Shelley S. Magill, Joseph D. Lutgring
| Article suivant Article suivant
  • Antibiotic use in tertiary care hospitals in Bangladesh: Revealing the extent through a point prevalence survey
  • Shariful Amin Sumon, Supta Sarker, A.B.M. Alauddin Chowdhury, Syed Abul Hassan Md. Abdullah, Md. Shahjahan, Sabrina Sharmin, Md. Golam Dostogir Harun

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

Déjà abonné à cette revue ?

Accès rapides

Mon compte

Aide & support

Plateformes Elsevier Masson

Déclaration CNIL

EM-CONSULTE.COM est déclaré à la CNIL, déclaration n° 1286925.

En application de la loi nº78-17 du 6 janvier 1978 relative à l'informatique, aux fichiers et aux libertés, vous disposez des droits d'opposition (art.26 de la loi), d'accès (art.34 à 38 de la loi), et de rectification (art.36 de la loi) des données vous concernant. Ainsi, vous pouvez exiger que soient rectifiées, complétées, clarifiées, mises à jour ou effacées les informations vous concernant qui sont inexactes, incomplètes, équivoques, périmées ou dont la collecte ou l'utilisation ou la conservation est interdite.
Les informations personnelles concernant les visiteurs de notre site, y compris leur identité, sont confidentielles.
Le responsable du site s'engage sur l'honneur à respecter les conditions légales de confidentialité applicables en France et à ne pas divulguer ces informations à des tiers.

Tout le contenu de ce site: Copyright © 2024 Elsevier, ses concédants de licence et ses contributeurs. Tout les droits sont réservés, y compris ceux relatifs à l'exploration de textes et de données, a la formation en IA et aux technologies similaires. Pour tout contenu en libre accès, les conditions de licence Creative Commons s'appliquent.