Comprehensive pharmacological and experimental study of Ginsenoside Re as a potential therapeutic agent for non-alcoholic fatty liver disease - 23/07/24
, Mingfei Duan b, Shaohong Wu c, Shan Jiang c, Songhao Hu a, Wenhui Chen a, Junchang Zhang a, Haiyan Quan d, ⁎ , Wah Yang a, ⁎ , Cunchuan Wang a, ⁎ on behalf of Global Obesity Collaborative
Abstract |
Objective |
Ginsenoside Re, a unique tetracyclic triterpenoid compound found in ginseng, has been suggested in previous reports to improve non-alcoholic fatty liver disease (NAFLD) by modulating lipid imbalance. This study aims to elucidate the potential mechanisms of Ginsenoside Re in treating NAFLD through a combination of bioinformatics analysis and biological experiments.
Methods |
Network pharmacology methods were employed to systematically depict the effective components and mechanisms of Ginsenoside Re in improving NAFLD. Molecular docking was utilized to evaluate the binding affinity of Ginsenoside Re with NAFLD-related targets and identify potential targets. NAFLD-related target genes were obtained from the GEO database for gene enrichment analysis, revealing signaling pathways, biological processes, and gene differential expression. Finally, animal experiments were conducted to verify the mechanism of action of Ginsenoside Re in NAFLD.
Results |
Network pharmacology analysis revealed that Ginsenoside Re improves NAFLD by modulating targets such as AKT1 and TLR4, findings corroborated by molecular docking, GEO database analysis, and experimental validation. Further investigation found that Ginsenoside Re ameliorates lipid metabolism disorders and inflammatory responses induced by NAFLD by modulating the PI3K/AKT and TLR4/NF-κB signaling pathways.
Conclusion |
Our study demonstrates the pharmacological effects of Ginsenoside Re in treating NAFLD, implicating multiple components, targets, and pathways. This provides a solid foundation for considering Ginsenoside Re as an alternative therapy for NAFLD, with promising clinical applications.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlight |
• | Network pharmacology analysis reveals the biological functions and signaling pathways of potential and core targets of Ginsenoside Re for NAFLD. |
• | Molecular docking technology predicts the affinity and binding modes of Ginsenoside Re with key targets of NAFLD. |
• | Based on the GEO database, prediction of the biological functions and signaling pathways of core genes is conducted, along with analysis of gene differential expression. |
• | Further experiments demonstrate that the therapeutic effect of Ginsenoside Re in improving NAFLD is closely associated with the regulation of lipid metabolism and inflammatory responses mediated by the PI3K/AKT and TLR4/NFκB pathways. |
Abbreviations : NAFLD, PPI, NCD, HFD, GO, KEGG, DEGs, TG, FFA, CHO, AST, ALT, HDL-C, LDL-C, PI3K, AKT, ACC, PPARγ, SREBP1c, FASN, TLR4, NF-κB, TNFα
Keywords : Ginsenoside Re, Non-alcoholic fatty liver disease, Network pharmacology, Molecular docking, Hepatic lipid accumulation
Plan
Vol 177
Article 116955- août 2024 Retour au numéro
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