Dendrobium nobile active ingredient Dendrobin A against hepatocellular carcinoma via inhibiting nuclear factor kappa-B signaling - 23/07/24
, Shaojiang Zheng a, ⁎ , Wei Jie a, ⁎ Abstract |
Objective |
Dendrobin A, a typical active ingredient of the traditional Chinese medicine Dendrobium nobile, has potential clinical application in cancer treatment; however, its effect and mechanism in anti-hepatocellular carcinoma (HCC) remain unsolved.
Method |
The effects of Dendrobin A on the viability, migration, invasion, cycle, apoptosis, and epithelial-mesenchymal transition of HepG2 and SK-HEP-1 cells were verified by in vitro experiments. mRNA sequencing was performed to screen the differentially expressed genes (DEGs) of HCC cells before and after Dendrobin A treatment, following GO enrichment and KEGG signaling pathway analyses. Mechanistically, molecular docking was used to evaluate the binding of Dendrobin A with proteins p65 and p50, before further verifying the activation of nuclear factor kappa-B (NF-κB) signaling. Finally, the antiproliferative effect of Dendrobin A on HCC cells was explored through animal experiments.
Results |
Dendrobin A arrested cell cycle, induced apoptosis, and inhibited proliferation, migration, invasion, and blocked epithelial-mesenchymal transition in HepG2 and SK-HEP-1 cells. mRNA sequencing identified 830 DEGs, involving various biological processes. KEGG analysis highlighted NF-κB signaling. Molecular docking revealed strong binding of Dendrobin A with p65 and p50 proteins, and western blotting confirmed reduced levels of p-p65 and p-p50 in HCC cells post Dendrobin A treatment. NF-κB agonist PMA reversed Dendrobin A-inhibited cell proliferation migration and invasion. In vivo experiments showed that Dendrobin A inhibited HCC cell growth.
Conclusion |
Our findings suggest that Dendrobin A exhibits anti-HCC properties by inhibiting the activation of the NF-κB pathway. These results provide a scientific basis for utilizing Dendrobium nobile in anti-HCC therapies.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
The active compound Dendrobin A, derived from Dendrobium nobile, exhibits significant anti-hepatocellular carcinoma effects. Its anti-cancer mechanism mainly involves inhibiting the NF-κB signaling pathway to suppress tumor cell proliferation, migration, invasion, epithelial-mesenchymal transition, block cell cycle progression, and induce cell apoptosis.
The active compound Dendrobin A, derived from Dendrobium nobile, exhibits significant anti-hepatocellular carcinoma effects. Its anti-cancer mechanism mainly involves inhibiting the NF-κB signaling pathway to suppress tumor cell proliferation, migration, invasion, epithelial-mesenchymal transition, block cell cycle progression, and induce cell apoptosis.Le texte complet de cet article est disponible en PDF.
Highlights |
• | Dendrobine A from Dendrobium nobile shows promise as an anti-HCC agent due to its multiple anti-cancer properties. |
• | Dendrobine A inhibits the proliferation of HCC cells by arresting the cell cycle at the G2-M phase. |
• | Dendrobine A induces apoptosis and inhibits the epithelial-mesenchymal transition of HCC cells. |
• | The anti-HCC effects of Dendrobine A are related to its inhibition of the activation of NF-κB signaling. |
Keywords : Hepatocellular carcinoma, Dendrobin A, Biological function, mRNA sequencing, NF-κB pathway
Plan
Vol 177
Article 117013- août 2024 Retour au numéro
Article précédent
- Preclinical in vivo assessment of the activity of AZD7442 anti-SARS-CoV-2 monoclonal antibodies against Omicron sublineages
- Jean-Sélim Driouich, Maxime Cochin, Guillaume Lingas, Léa Luciani, Cécile Baronti, Ornéllie Bernadin, Magali Gilles, Paola Mariela Saba Villarroel, Grégory Moureau, Paul-Rémi Petit, Axelle Dupont, Jacques Izopet, Nassim Kamar, Brigitte Autran, Gilles Paintaud, Sophie Caillard, Amandine le Bourgeois, Christophe Richez, Lionel Couzi, Aliénor Xhaard, Zora Marjanovic, Jerome Avouac, Caroline Jacquet, Dany Anglicheau, Morgane Cheminant, Stéphanie Nguyen, Benjamin Terrier, Jacques Eric Gottenberg, Caroline Besson, Sophie Letrou, Josephine Tine, Joe Miantezila Basilua, Denis Angoulvant, Coralie Tardivon, Gilles Blancho, Guillaume Martin-Blondel, Yazdan Yazdanpanah, France Mentré, Vincent Lévy, Franck Touret, Jérémie Guedj, Xavier de Lamballerie, Antoine Nougairède
- Brusatol alleviates pancreatic carcinogenesis via targeting NLRP3 in transgenic Krastm4Tyj Trp53tm1Brn Tg (Pdx1-cre/Esr1*) #Dam mice
- Juan Zhang, Yu-Lin Wu, Hong-Xi Xu, Yi-Bo Zhang, Pei-Yao Ren, Yan-Fang Xian, Zhi-Xiu Lin
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?