Modified Zhizhu Pill improves the loperamide-induced slow transit constipation via gut microbiota and neurotransmitters in microbiota-gut-brain axis - 10/07/24
Highlights |
• | Modified Zhizhu Pill had a mitigating effect on loperamide-induced constipation in rats. |
• | Modified Zhizhu Pill ameliorated the microecological dysbiosis of the intestinal microbiota. |
• | Modified Zhizhu Pill improved neurotransmitters. |
• | Modified Zhizhu Pill involved in microbiota-gut-brain axis. |
Abstract |
Background |
Slow-transmission constipation is a type of intractable constipation with unknown etiology and unclear pathogenesis.
Objective |
The intention of this study was to evaluate the therapeutic effect and possible mechanism of Modified Zhizhu Pills on loperamide-induced slow transit constipation.
Methods |
The effects of the Modified Zhizhu Pill were evaluated in a rat model of constipation induced by subcutaneous administration of loperamide. Fecal parameters (fecal count, fecal water content, and fecal hardness) were measured in constipated rats. The substance, target, and pathway basis of the Modified Zhizhu Pill on constipation was investigated using network pharmacology. The microflora in rats was determined. Serum neurotransmitters (acetylcholine and 5-hydroxytryptamine) were measured in rats and their relationship with the gut microbiota was assessed.
Results |
Modified Zhizhu Pill increased the number of bowel movements and fecal water content, and decreased fecal hardness and transit time. Network pharmacological analysis showed that Modified Zhizhu Pill can target multiple constipation-related targets and pathways through multiple potential active ingredients. Modified Zhizhu Pill alleviated loperamide-induced microbiota dysbiosis. Modified Zhizhu Pill increased serum 5-hydroxytryptamine and acetylcholine. The increase in serum 5-hydroxytryptamine and acetylcholine was associated with rat gut microbiota.
Conclusion |
These results suggest that Modified Zhizhu Pill may increase intestinal motility and ultimately relieve constipation by improving microecological dysbiosis and neurotransmission.
Le texte complet de cet article est disponible en PDF.Keywords : Constipation, Herbal formula, Gut microbiota, Acetylcholine, 5-hydroxytryptamine
Plan
Vol 48 - N° 7
Article 102410- août 2024 Retour au numéro
Article précédent
- HSP70 protects against acute pancreatitis-elicited intestinal barrier damage in rats
- Sijin Chen, Rong Qin, Zhibo Zhang, Xirui Fan, Lifang Zhou, Hui Wang
- Selective inner muscle layer myotomy is associated with lower pain and same clinical efficacy that full-thickness myotomy in patients treated by POEM for achalasia: A multicenter retrospective comparative analysis of 158 patients
- Mathilde Sanavio, Blandine Vauquelin, Marie-Christine Picot, Romain Altwegg, Anne Bozon, Flora Charpy, Ludovic Caillo, Arthur Berger, Frank Zerbib, Antoine Debourdeau
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?