Clinical significance of lncRNA XIST expression in cholangiocarcinoma and its effect on cell migration and invasion - 28/06/24

Doi : 10.1016/j.clinre.2024.102398

Chenxi Li ^a,^{^{#
These two authors contributed equally to the study}}, Yifei Dong ^b,^{^{#
These two authors contributed equally to the study}}, Yichuan Zhang ^c,^⁎ , Caihong Wu ^d,^e,^f,^⁎
^a Department of General Surgery, Wenling Hospital of Traditional Chinese Medicine, Wenling 317500, China
^b Department of Laboratory, The Eighth Hospital of Wuhan, Wuhan 430010, China
^c Minimally Invasive Endoscopy Center, Digestive Disease Center, The Affiliated Hospital of Panzhihua University, Panzhihua 617000, China
^d Department of Anesthesiology, The Second Xiangya Hospital of Central South University, Changsha 421001, China
^e Anesthesia Medical Research Center of Central South University, Changsha 421001, China
^f Department of Clinical Nursing, The Second Xiangya Hospital of Central South University, Changsha 421001, China

^⁎⁎Corresponding author at: Department of Anesthesiology, The Second Xiangya Hospital of Central South University; Anesthesia Medical Research Center of Central South University; Department of Clinical Nursing, The Second Xiangya Hospital of Central South University, No. 139, Renmin Middle Road, Furong District, Changsha 421001, China.Department of AnesthesiologyThe Second Xiangya Hospital of Central South UniversityAnesthesia Medical Research Center of Central South UniversityDepartment of Clinical NursingThe Second Xiangya Hospital of Central South UniversityNo. 139, Renmin Middle Road, Furong DistrictChangsha421001China^⁎Corresponding author at: Minimally Invasive Endoscopy Center, Digestive Disease Center, The Affiliated Hospital of Panzhihua University, 27 Taoyuan Street, East District, Panzhihua 617000, China.Minimally Invasive Endoscopy CenterDigestive Disease CenterThe Affiliated Hospital of Panzhihua University27 Taoyuan Street, East DistrictPanzhihua617000China

Hightlights

•	XIST may serve as a prognostic biomarker for cholangiocarcinoma.
•	Overexpression of XIST inhibited tumor progression by targeting miR-126–3p.
•	XIST has a carcinogenic role in cholangiocarcinoma.

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Abstract

Background

Cholangiocarcinoma is a malignant tumor that occurs in the bile duct system, and the prognosis of patients is poor. Currently, research suggests that long non-coding RNAs (lncRNAs) in the treatment and prevention of cholangiocarcinoma. This study primarily focuses on the regulation and potential mechanism of the lncRNA XIST (XIST) in cholangiocarcinoma.

Methods

The levels of XIST and miR-126–3p in cholangiocarcinoma tissues and cells were detected using real-time quantitative polymerase chain reaction (RT-qPCR). Cell transfection status, including migration and invasion, was examined via the Transwell method. The relationship between XIST and miR-126–3p was observed by dual-luciferase gene reporter assay and verified by rescue assays. Additionally, the prognostic significance of XIST in cholangiocarcinoma was determined using Kaplan-Meier and multivariate Cox regression analyses.

Results

XIST expression was increased in cholangiocarcinoma, while miR-126–3p was decreased, in both tissues and cells. The successful construction of silencing XIST was found to inhibit the count of cell migration and invasion. XIST directly targeted miR-126–3p to regulate the progression of cholangiocarcinoma.

Conclusion

XIST sponging miR-126–3p inhibited the progression of cholangiocarcinoma and improved the prognosis for patients. This finding provides new insights and opportunities for future studies on cholangiocarcinoma prognostic biomarkers.

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Keywords : lncRNA XIST, miR-126–3p, Cholangiocarcinoma, Prognostic biomarker

Plan

Introduction

Materials and methods

Patient enrollment and tissue collection

Cultivation of cholangiocarcinoma cells

RT-qPCR analysis

Cell transfection, migration and invasion assays

Dual-luciferase reporter assay

Statistical analysis

Results

Expression of lncRNA XIST and its relationship with cholangiocarcinoma patients

High expression of XIST suggested poor prognosis in cholangiocarcinoma patients

Effect of XIST low expression on cholangiocarcinoma cells

XIST targeted miR-126–3p in cholangiocarcinoma

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Vol 48 - N° 7

Article 102398- août 2024 Retour au numéro

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Clinical significance of lncRNA XIST expression in cholangiocarcinoma and its effect on cell migration and invasion - 28/06/24

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