Investigation of lactotransferrin messenger RNA expression levels as an anti–type 2 asthma biomarker - 13/06/24
![](/templates/common/images/mail.png)
Abstract |
Background |
Lactotransferrin (LTF) has an immunomodulatory function, and its expression levels are associated with asthma susceptibility.
Objectives |
We sought to investigate LTF messenger RNA (mRNA) expression levels in human bronchial epithelial cells (BECs) as an anti–type 2 (T2) asthma biomarker.
Methods |
Association analyses between LTF mRNA expression levels in BECs and asthma-related phenotypes were performed in the Severe Asthma Research Program (SARP) cross-sectional (n = 155) and longitudinal (n = 156) cohorts using a generalized linear model. Correlation analyses of mRNA expression levels between LTF and all other genes were performed by Spearman correlation.
Results |
Low LTF mRNA expression levels were associated with asthma susceptibility and severity (P < .025), retrospective and prospective asthma exacerbations, and low lung function (P < 8.3 × 10−3). Low LTF mRNA expression levels were associated with high airway T2 inflammation biomarkers (sputum eosinophils and fractional exhaled nitric oxide; P < 8.3 × 10−3) but were not associated with blood eosinophils or total serum IgE. LTF mRNA expression levels were negatively correlated with expression levels of TH2 or asthma-associated genes (POSTN, NOS2, and MUC5AC) and eosinophil-related genes (IL1RL1, CCL26, and IKZF2) and positively correlated with expression levels of TH1 and inflammation genes (IL12A, MUC5B, and CC16) and TH17-driven cytokines or chemokines for neutrophils (CXCL1, CXCL6, and CSF3) (P < 3.5 × 10−6).
Conclusions |
Low LTF mRNA expression levels in BECs are associated with asthma susceptibility, severity, and exacerbations through upregulation of airway T2 inflammation. LTF is a potential anti-T2 biomarker, and its expression levels may help determine the balance of eosinophilic and neutrophilic asthma.
Le texte complet de cet article est disponible en PDF.Key words : Anti-T2 biomarker, asthma exacerbations, asthma susceptibility, bronchial epithelial cells, eosinophils, lactotransferrin, mRNA expression, severe asthma, neutrophils, T2 inflammation
Abbreviations used : BD, BEC, BMI, Feno, FEV1, FVC, GTEx Portal, GWAS Catalog, LTF, mRNA, MTEC, OR, RNA-Seq, SARP, SNP, T2, WT
Plan
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?