Omadacycline drug susceptibility testing for non-tuberculous mycobacteria using oxyrase to overcome challenges with drug degradation - 10/06/24
, Sanjay Singh a, Pamela J. McShane d, Julie V. Philley d, Shashikant Srivastava a, e, ⁎ Abstract |
Background |
Drug susceptibility testing (DST) protocol of omadacycline against non-tuberculous mycobacteria has not yet been established. We developed a method to accurately determine MIC omadacycline MIC against Mycobacterium abscessus (Mab), Mycobacterium avium-complex (MAC), and Mycobacterium kansasii (Mkn).
Methods |
First, we identified the oxyrase concentration not affecting Mab, MAC, and Mkn growth followed by omadacycline MIC experiments with and without oxyrase using reference and clinical strains.
Results |
Oxyrase 0.5 % (v/v) stabilized omadacycline in the culture medium. The median omadacycline MIC was 1 mg/L for Mab and 8 mg/L for Mkn. For MAC, the median omadacycline MIC was 2 mg/L for M. avium, 256 mg/L for M. intracellulare, and 4 mg/L for M. chimaera (p < 0.0001). Wilcoxon matched-pairs signed rank test revealed statistically lower MICs with oxyrase for all MAC subspecies (p < 0.0001), all Mab subspecies (p < 0.0001), and Mkn (p = 0.0002). The decrease in MICs with oxyrase was 17/18 of Mab, 14/19 of Mkn, 8/8 of M. avium, 4/5 M. chimera, but only 11/18 of M. intracellulare (p < 0.013).
Conclusion |
Use of 0.5 % oxyrase could be a potential solution to reliable and reproducible omadacycline MIC of Mab. However, oxyrase demonstrated a variable effect in reducing MICs against MAC and Mkn.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Oxyrase prevents omadacycline degradation in solution. |
• | Use of 0.5 % oxyrase could be a potential solution to reliable and reproducible omadacycline MIC of M. abscessus. |
• | Oxyrase itself demonstrated antimicrobial effect against slow-growing mycobacteria and variable effect in reducing SGM's MICs. |
Keywords : Mycobacterium avium-complex, Mycobacterium kansasii, Mycobacterium abscessus, Oxyrase, Minimum inhibitory concentration
Plan
Vol 147
Article 102519- juillet 2024 Retour au numéro
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