Epithelial overexpression of IL-33 induces eosinophilic esophagitis dependent on IL-13 - 03/05/24
Graphical abstract |
Abstract |
Background |
Eosinophilic esophagitis (EoE) is an increasingly common inflammatory condition of the esophagus; however, the underlying immunologic mechanisms remain poorly understood. The epithelium-derived cytokine IL-33 is associated with type 2 immune responses and elevated in esophageal biopsy specimens from patients with EoE.
Objective |
We hypothesized that overexpression of IL-33 by the esophageal epithelium would promote the immunopathology of EoE.
Methods |
We evaluated the functional consequences of esophageal epithelial overexpression of a secreted and active form of IL-33 in a novel transgenic mouse, EoE33. EoE33 mice were analyzed for clinical and immunologic phenotypes. Esophageal contractility was assessed. Epithelial cytokine responses were analyzed in three-dimensional organoids. EoE33 phenotypes were further characterized in ST2−/−, eosinophil-deficient, and IL-13−/− mice. Finally, EoE33 mice were treated with dexamethasone.
Results |
EoE33 mice displayed ST2-dependent, EoE-like pathology and failed to thrive. Esophageal tissue remodeling and inflammation included basal zone hyperplasia, eosinophilia, mast cells, and TH2 cells. Marked increases in levels of type 2 cytokines, including IL-13, and molecules associated with immune responses and tissue remodeling were observed. Esophageal organoids suggested reactive epithelial changes. Genetic deletion of IL-13 in EoE33 mice abrogated pathologic changes in vivo. EoE33 mice were responsive to steroids.
Conclusions |
IL-33 overexpression by the esophageal epithelium generated immunopathology and clinical phenotypes resembling human EoE. IL-33 may play a pivotal role in the etiology of EoE by activating the IL-13 pathway. EoE33 mice are a robust experimental platform for mechanistic investigation and translational discovery.
Le texte complet de cet article est disponible en PDF.Key words : IL-33, eosinophilic esophagitis, transgene, eosinophil, type 2 inflammation
Abbreviations used : a.a, BZH, CAE, DEG, EoE, EoEHSS, EPX, FC, H&E, IHC, MCh, OVA, RNA sequencing, saIL-33, WT
Plan
Vol 153 - N° 5
P. 1355-1368 - mai 2024 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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