Theabrownin from Qingzhuan tea prevents high-fat diet-induced MASLD via regulating intestinal microbiota - 27/04/24

Doi : 10.1016/j.biopha.2024.116582

Yong Chen ^a,^b,^{^{1
Yong Chen and Chen Xie contributed equally to this work and both are co-first authors.}}, Chen Xie ^a,^b,^c,^{^{1
Yong Chen and Chen Xie contributed equally to this work and both are co-first authors.}}, Yining Lei ^a, Dan Ye ^a,^b, Le Wang ^a,^b, Fang Xiong ^a,^b, Hui Wu ^d, Qiang He ^d, Hongfu Zhou ^a,^b, Ling Li ^a,^b, Jun Xing ^a,^b, Cai Wang ^a,^b, Min Zheng ^a,^b,^⁎
^a Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, China
^b Hubei Industrial Technology Research Institute of Intelligent Health, Xianning 437100, China
^c Obstetrics and Gynecology of the Second Affiliated Hospital of Hubei University of Science and Technology, Xianning 437100, China
^d Xianning Public Inspection Center of Hubei Province, Xianning 437100, China

^⁎Corresponding author at: Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, China.Xianning Medical College, Hubei University of Science and TechnologyXianning437100China.

Abstract

The aim of this study was to investigate whether the therapeutic effect of theabrownin extracted from Qingzhuan tea (QTB) on metabolic dysfunction-associated steatosis liver disease (MASLD) is related to the regulation of intestinal microbiota and its metabolite short-chain fatty acids (SCFAs). Mice were divided into four groups and received normal diet (ND), high-fat diet (HFD) and HFD+QTB (180, 360 mg/kg) for 8 weeks. The results showed that QTB significantly reduced the body weight of HFD mice, ameliorated liver lipid and dyslipidemia, and increased the level of intestinal SCFAs in HFD mice. The results of 16 S rRNA showed that the relative abundance of Bacteroides, Blautia and Lachnoclostridium and their main metabolites acetate and propionate were significantly increased after QTB intervention. The relative abundance of Colidextribacter, Faecalibaculum and Lactobacillus was significantly reduced. QTB can also significantly up-regulate the expression of ATGL, PPARα, FFAR2 and FFAR3, and inhibit the expression of LXRα, SREBP-1c, FAS and HMGCR genes. This makes it possible to act as a prebiotic to prevent MASLD.

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Graphical Abstract

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Highlights

•	The therapeutic effect of theabrownin on MASLD was demonstrated.
•	Regulating intestinal microbiota and its metabolites in mice.
•	The SCFAs receptors may be potential therapeutic targets for MASLD.

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Keywords : Theabrownin, MASLD, Obesity, Intestinal microbiota, Short-chain fatty acids

Plan

Introduction

Materials and methods

Preparation and content determination of QTB

Animal experiments

Biochemical analysis

Histological and morphometry analysis

Intestinal microbiota analysis

SCFAs in feces analysis

RT‑PCR

Statistical analysis

Results

QTB reduced body weight and regulated lipid abnormalities in HFD-induced mice

QTB ameliorated hepatic steatosis in mice

QTB reduced adipose tissue accumulation in HFD-fed mice

QTB modulated intestinal microbiota and SCFAs in mice

QTB altered the metabolic gene expressions of liver

Discussion

CRediT authorship contribution statement

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Vol 174

Article 116582- mai 2024 Retour au numéro

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Theabrownin from Qingzhuan tea prevents high-fat diet-induced MASLD via regulating intestinal microbiota - 27/04/24

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