Oleanolic acid exerts bone anabolic effects via activation of osteoblastic 25-hydroxyvitamin D 1-alpha hydroxylase - 22/03/24
Abstract |
Oleanolic acid (OA) is previously shown to exert bone protective effects in aged animals. However, its role in regulating osteoblastic vitamin D bioactivation, which is one of major causes of age-related bone loss, remains unclear. Our results revealed that treatment of OA significantly increased skeletal CYP27B1 expression and circulating 1,25(OH)2D3 in ovariectomized mice (p <0.01). Moreover, OA upregulated CYP27B1 protein expression and activity, as well as the vitamin D-responsive bone markers alkaline phosphatase (ALP) activity and osteopontin (OPN) protein expression, in human osteoblast-like MG-63 cells (p<0.05). CYP27B1 expression increased along with the osteoblastic differentiation of human bone marrow derived mesenchymal stem cells (hMSCs). CYP27B1 expression and cellular 1,25(OH)2D3 production were further potentiated by OA in cells at mature osteogenic stages. Notably, our study suggested that the osteogenic actions of OA were CYP27B1 dependent. In summary, the bone protective effects of OA were associated with the induction of CYP27B1 activity and expression in bone tissues and osteoblastic lineages. Hence, OA might be a potential approach for management of age-related bone loss.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Highlights |
• | Oleanolic acid (OA) upregulated CYP27B1 expression in bone, which is positively correlated with bone anabolic process. |
• | OA improved CYP27B1 activity and osteogenesis of human mesenchymal stem cells derived from older adults. |
• | OA might serve as a potential approach for management of age-related bone loss and vitamin D metabolism in elderly. |
Keywords : 25-Hydroxyvitamin D3 1-alpha-hydroxylase, Vitamin D, Osteoporosis, Oleanolic acid, Aging
Plan
Vol 173
Article 116402- avril 2024 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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