Attenuation of carbon tetrachloride-induced nephrotoxicity by gum Arabic extract via modulating cellular redox state, NF-κB pathway, and KIM-1 - 22/03/24
Abstract |
The current study investigated the ameliorating impact of GA water extract (GAE) on CCl4-induced nephrotoxicity in renal cells and tissue by comparing its effectiveness with the Ketosteril (Ks) drug in restoring oxidative stress and necroinflammation. The cell morphology, necrosis, and redox state were evaluated in Vero cells. The influence of GAE on CCl4-induced oxidative stress, inflammation, and necrosis was examined in rats. The predicted inhibitory mechanism of GAE phenolic constituents against COX-2 and iNOS was also studied. The results revealed that GAE contains crucial types of phenolic acids, which are associated with its antiradical activities. GAE improved CCl4-induced Vero cell damage and restored renal architecture damage, total antioxidant capacity, ROS, TBARS, NO, GSH, GPX, SOD, and MPO in rats. GAE downregulated the gene expression of renal NF-κB, TNF-α, iNOS, and COX-2, as well as kidney injury molecule-1 (KIM-1) in rats. The GAE improved blood urea, creatinine, cholesterol, and reducing power. The computational analysis revealed the competitive inhibitory mechanism of selected phenolic composites of GAE on COX-2 and iNOS activities. The GAE exhibited higher potency than Ks in most of the studied parameters, as observed by the heatmap plots. Thus, GAE is a promising extract for the treatment of kidney toxicity.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | GAE is the water extract of gum Arabic. |
• | GAE showed an attenuation effect on CCl4-induced nephrotoxicity in Vero cells. |
• | GAE showed an attenuation effect on CCl4-induced nephrotoxicity in rats. |
• | GAE improved renal oxidative stress, necroinflammation, and morphology. |
• | GAE had higher anti-nephrotoxicity impact than Ketostreil alpha-ketoanalogue drug. |
Keywords : Gum Arabic extract, CCl4, Nephrotoxicity, Oxidative stress, Necroinflammation, Computational analysis
Plan
Vol 173
Article 116340- avril 2024 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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