Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis - 22/03/24
Abstract |
Glycyrrhetinic acid (GA) shows great efficiency against non-small cell lung cancer (NSCLC), but the detailed mechanism is unclear, which has limited its clinical application. Herein, we investigated the potential targets of GA against NSCLC by activity-based protein profiling (ABPP) technology and the combination of histopathology and proteomics validation. In vitro and in vivo results indicated GA significantly inhibited NSCLC via promotion of peroxiredoxin-6 (Prdx6) and caspase-3 (Casp3)-mediated mitochondrial apoptosis. This original finding will provide theoretical and data support to improve the treatment of NSCLC with the application of GA.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Structural illustration of glycyrrhetinic acid inhibiting non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis detected by activity-based protein profiling technology and the combination of histopathology and proteomics validation.
Structural illustration of glycyrrhetinic acid inhibiting non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis detected by activity-based protein profiling technology and the combination of histopathology and proteomics validation.Le texte complet de cet article est disponible en PDF.
Highlights |
• | First-time integrated analysis profiling of non-small cell lung cancer (NSCLC) and Glycyrrhetinic acid (GA) treatment by integrating activity-based protein profiling (ABPP) technology, histopathology and proteomics validation both in vitro and in vivo. |
• | Identification of GA promoted ROS accumulation and inhibited proliferation of NSCLC cells. |
• | Identification of Casp3 and Prdx6 were direct target proteins of GA in the treatment of NSCLC cells. |
Abbreviations : ABPP, ATP, BCA, Casp3, CCK8, CETSA-WB, DCFH-DA, DEGs, DEPs, DMEM, DMSO, DTT, FBS, GA, GAPDH, GO, HE, IAA, IAA-yne, KEGG, LC-MS, LLC, NSCLC, PBS, Prdx6, ROS, TBTA, TCEP, TEAB
Keywords : Glycyrrhetinic acid, NSCLC, Activity-based protein profiling
Plan
Vol 173
Article 116304- avril 2024 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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