3D printed PLGA scaffold with nano-hydroxyapatite carrying linezolid for treatment of infected bone defects - 29/02/24
, Yuan quan Wu a, ⁎ Abstract |
Background |
Linezolid has been reported to protect against chronic bone and joint infection. In this study, linezolid was loaded into the 3D printed poly (lactic-co-glycolic acid) (PLGA) scaffold with nano-hydroxyapatite (HA) to explore the effect of this composite scaffold on infected bone defect (IBD).
Methods |
PLGA scaffolds were produced using the 3D printing method. Drug release of linezolid was analyzed by elution and high-performance liquid chromatography assay. PLGA, PLGA-HA, and linezolid-loaded PLGA-HA scaffolds, were implanted into the defect site of a rabbit radius defect model. Micro-CT, H&E, and Masson staining, and immunohistochemistry were performed to analyze bone infection and bone healing. Evaluation of viable bacteria was performed. The cytocompatibility of 3D-printed composite scaffolds in vitro was detected using human bone marrow mesenchymal stem cells (BMSCs). Long-term safety of the scaffolds in rabbits was evaluated.
Results |
The linezolid-loaded PLGA-HA scaffolds exhibited a sustained release of linezolid and showed significant antibacterial effects. In the IBD rabbit models implanted with the scaffolds, the linezolid-loaded PLGA-HA scaffolds promoted bone healing and attenuated bone infection. The PLGA-HA scaffolds carrying linezolid upregulated the expression of osteogenic genes including collagen I, runt-related transcription factor 2, and osteocalcin. The linezolid-loaded PLGA-HA scaffolds promoted the proliferation and osteogenesis of BMSCs in vitro via the PI3K/AKT pathway. Moreover, the rabbits implanted with the linezolid-loaded scaffolds showed normal biochemical profiles and normal histology, which suggested the safety of the linezolid-loaded scaffolds.
Conclusion |
Overall, the linezolid-loaded PLGA-HA scaffolds fabricated by 3D printing exerts significant bone repair and anti-infection effects.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Linezolid-loaded PLGA-HA scaffolds exhibited a sustained release of linezolid. |
• | Linezolid-loaded PLGA-HA scaffolds promoted bone healing in rabbit radius defect model. |
• | Linezolid-loaded PLGA-HA scaffolds prevented infection in vivo. |
• | Linezolid-loaded PLGA-HA scaffolds exhibited good biocompatibility and promoted osteogenesis. |
Keywords : Linezolid, PLGA-HA, Scaffolds, Bone defects, Bone regeneration, Infection
Plan
Vol 172
Article 116228- mars 2024 Retour au numéro
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