Label-free analysis of the β-hydroxybutyricacid drug on mitochondrial redox states repairment in type 2 diabetic mice by resonance raman scattering - 29/02/24
Abstract |
Background |
Mitochondrial redox imbalance underlies the pathophysiology of type2 diabetes mellitus (T2DM), and is closely related to tissue damage and dysfunction. Studies have shown the beneficial effects of dietary strategies that elevate β-hydroxybutyrate (BHB) levels in alleviating T2DM. Nevertheless, the role of BHB has not been clearly elucidated.
Methods |
We performed a spectral study to visualize the preventive effects of BHB on blood and multiorgan mitochondrial redox imbalance in T2DM mice via using label-free resonance Raman spectroscopy (RRS), and further explored the impact of BHB therapy on the pathology of T2DM mice by histological and biochemical analyses.
Findings |
Our data revealed that RRS-based mitochondrial redox states assay enabled clear and reliable identification of the improvement of mitochondrial redox imbalance by BHB, evidenced by the reduction of Raman peak intensity at 750 cm−1, 1128 cm−1 and 1585 cm−1 in blood, tissue as well as purified mitochondria of db/db mice and the increase of tissue mitochondrial succinic dehydrogenase (SDH) staining after BHB treatment. Exogenous supplementation of BHB was also found to attenuate T2DM pathology related to mitochondrial redox states, involving organ injury, blood glucose control, insulin resistance and systemic inflammation.
Interpretation |
Our findings provide strong evidence for BHB as a potential therapeutic strategy targeting mitochondria for T2DM.
Le texte complet de cet article est disponible en PDF.Highlights |
• | First use of RRS to investigate BHB's impact on mitochondrial redox in T2DM mice. |
• | Assessed mitochondrial redox in blood and tissues using Raman peaks at 750 cm−1 and 1128 cm−1. |
• | RRS results indicate that BHB can improve mitochondrial function in T2DM mice. |
• | Administration of BHB also attenuates T2DM pathology related to mitochondrial dysfunction. |
Keywords : Type 2 diabetes, β-hydroxybutyric acid, Mitochondrial redox state, Raman spectroscopy, Label-free
Plan
Vol 172
Article 116320- mars 2024 Retour au numéro
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