Gut microbiota-based discovery of Houttuyniae Herba as a novel prebiotic of Bacteroides thetaiotaomicron with anti-colitis activity - 29/02/24
Abstract |
Ulcerative colitis (UC) represents an inflammatory disease characterized by fluctuations in severity, posing substantial challenges in treatment. The gut microbiota plays a pivotal role in the pathogenesis of UC. This study sought to identify drugs specifically targeting the gut microbiota to mitigate UC. We initiated a meta-analysis on gut microbiota in UC patients to identify UC-associated bacterial strains. Subsequently, we screened 164 dietary herbal medicines in vitro to identify potential prebiotics for the UC-associated bacterium, Bacteroides thetaiotaomicron. The DSS-induced colitis mouse model was utilized to evaluate the anti-colitis efficacy of the identified dietary herbal medicine. Full-length 16 S rRNA amplicon sequencing was employed to observe changes in gut microbiota following dietary herbal medicine intervention. The relative abundance of Bacteroides was notably diminished in UC patients compared to their healthy counterparts. B. thetaiotaomicron exhibited an inverse relationship with UC symptoms, indicating its potential as an anti-colitis agent. In vitro assessments revealed that H. Herba significantly bolstered the proliferation of B. thetaiotaomicron. Further experiments showed that treating DSS-induced mice with an aqueous extract of H. Herba considerably alleviated colitis indicators such as weight loss, colon shortening, disease activity score (DAI), and systemic inflammation. Microbial analysis revealed B. thetaiotaomicron as the sole bacterium substantially augmented by H. Herba in vivo. Overall H. Herba emerges as a promising prebiotic for B. thetaiotaomicron, offering significant anti-colitis benefits. Employing a gut microbiota-centric approach proves valuable in the quest for drug discovery.This study provides a new paradigm for drug discovery that targets the gut microbiota to treat UC.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | UC patients exhibit a significant reduction in Bacteroides genus. |
• | Bacteroides thetaiotaomicron demonstrates potent anti-UC properties. |
• | Houttuyniae Herba promotes the proliferation of B. thetaiotaomicron both in vitro and in vivo. |
• | Function foods or medicinal foods are regarded as new therapeutic strategies for UC. |
• | This study proposed a gut microbiota-centric approach for drug discovery. |
Keywords : Ulcerative colitis, Gut microbiota, Bacteroides thetaiotaomicron, Dietary herbal medicine, Houttuyniae Herba
Plan
Vol 172
Article 116302- mars 2024 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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