The multi-protective effect of IL-37-Smad3 against ox-LDL induced dysfunction of endothelial cells - 29/02/24
Abstract |
Atherosclerosis is a lipid-driven inflammatory arterial disease, with one crucial factor is oxidized low-density lipoprotein (ox-LDL), which can induce endothelial dysfunction through endoplasmic reticulum stress (ERS). Interleukin-37 (IL-37) exerts vascular protective functions. This study aims to investigates whether IL-37 can alleviate ERS and autophagy induced by ox-LDL, therely potentialy treating atherosclerosis. We found that ox-LDL enhances the wound healing rate in Rat Coronary Artery Endothelial Cells (RCAECs) and IL-37 reduce the ox-LDL-induced pro-osteogenic response, ERS, and autophagy by binding to Smad3. In RCAECs treated with ox-LDL and recombinant human IL-37, the wound healing rate was mitigated. The expression of osteogenic transcription factors and proteins involved in the ERS pathway was reduced in the group pretreated with IL-37 and ox-LDL. However, these responses were not alleviated when Smads silenced. Electron microscopy revealed that the IL-37/Smad3 complex could suppress endoplasmic reticulum autophagy under ox-LDL stimulation. Thus, IL-37 might treat atherosclerosis through its multi-protective effect by binding Smad3.
Le texte complet de cet article est disponible en PDF.Highlights |
• | IL-37, binding with Smad3, emerges as a potent defender against ox-LDL-induced endothelial dysfunction, showcasing diverse protective actions. |
• | The IL-37-Smad3 complex efficiently hinders ox-LDL-induced autophagy, and enhances endothelial function by modulating ER stress and osteogenic responses, unveiling a novel facet of IL-37's protective role in endothelial cells. |
• | Smad3 dependence underscores IL-37's unique anti-atherosclerotic mechanism, emphasizing its specificity in mediating protective effects. |
• | IL-37's pivotal role in mitigating ER stress emerges as a central aspect in its multifaceted protection against atherosclerosis, offering promising therapeutic avenues. |
Keywords : Interleukin-37, Ox-LDL, Endoplasmic reticulum stress (ERS), Smad3
Plan
Vol 172
Article 116268- mars 2024 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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