Understanding translational biochemistry of the skin is an essential component in mastering non-invasive aesthetic treatments. Collagen is the most abundant protein in the animal kingdom and plays a significant role in maintaining structural function in biologically healthy human skin. Collagen degradation and synthesis occurs throughout human life. Upregulation of collagen synthesis remains the mainstay of non-invasive aesthetic skin treatments. Elastin is a smaller yet significant component in the skin’s ability to maintain biologically healthy stretch and recoil. Multi-Omics represents a relatively nascent field in the optimization and development of therapies aimed at the aesthetic improvement of the skin.