Oral delivery of a highly stable superoxide dismutase as a skin aging inhibitor - 17/06/23

Doi : 10.1016/j.biopha.2023.114878

Liang Dong ^a,^e,^{^{1
These authors contributed equally to the work.}}, Yongli Chen ^a,^b,^{^{1
These authors contributed equally to the work.}}, Lihong Gu ^a,^b, Miao Gan ^a,^b, Andrew Carrier ^c, Ken Oakes ^d, Xu Zhang ^c,^⁎ , Zhiyang Dong ^a,^⁎
^a State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, P.O. Box 2714, Beijing 100080, China
^b Shenzhen Siyomicro BIO-TECH CO., Ltd., Shenzhen 518116, China
^c Department of Chemistry, Cape Breton University, 1250 Grand Lake Road, Sydney, Nova Scotia B1P 6L2, Canada
^d Department of Biology, Cape Breton University, 1250 Grand Lake Road, Sydney, Nova Scotia B1P 6L2, Canada
^e University of Chinese Academy of Sciences, Beijing 100049, China

^⁎Corresponding authors.

Abstract

As an effective antioxidant enzyme, superoxide dismutase (SOD) has been widely used as a food supplement, cosmetic additive, and therapeutic agent. However, oral delivery of SOD is challenging due to its relative instability, limited bioavailability, and low absorption efficiency in the gastrointestinal (GI) tract. We addressed these issues using a highly stable superoxide dismutase (hsSOD) generated from a hot spring microbial sample. This SOD exhibited a specific activity of 5000 IU/mg while retaining its enzymatic activity under low pH environments of an artificial GI system and in the presence of surfactants and various proteolytic enzymes. The inhibitory effects of hsSOD against skin-aging was evaluated under both in vitro and in vivo experiments using fibroblast cell and D-galactose induced aging-mouse models, respectively. Effective oral delivery of hsSOD promises wide applicability in pharmaceutical and food industries.

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Highlights

•	A highly stable SOD (hsSOD) enzyme proves feasible for oral administration.
•	High absorption and therapeutic efficacy were demonstrated.
•	The therapeutic hsSOD shows promise in the prevention of skin-aging.

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Abbreviations : SOD, hsSOD, ROS, GSH, MDA, HYP, SGF, SIF, SB, AST, ALT, UA, CREA, OS, GI, D-gal, EDTA, BSA, ABTS, NBT, SA-β-gal, IPTG, MWCO, Vit C, GAPDH

Keywords : Skin aging, Superoxide dismutase, Oral delivery, Antioxidant, Bioavailability

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Vol 164

Article 114878- août 2023 Retour au numéro

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Oral delivery of a highly stable superoxide dismutase as a skin aging inhibitor - 17/06/23

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