Pinostrobin alleviates testicular and spermatological damage induced by polystyrene microplastics in adult albino rats - 29/04/23
, Jeremiah Oshiomame Unuofin c, Sogolo Lucky Lebelo c, Jesus Simal-Gandara d, ⁎ Abstract |
Background |
Polystyrene microplastics (PS-MPs) have become major environmental pollutants that adversely effects multiple organs specifically testicles. Pinostrobin (PN) is an important flavonoid which, shows several pharmacological potentials.
Purpose |
The current study was designed to elucidate the mitigative effects of PN against PS-MPs induced testicular toxicities in rats.
Methods |
48 male albino rats were randomly distributed into 4 groups, control, PS-MPs group (0.01 mg/kg), PS-MPs + PN group (0.01 mg/kg of PS-MPs; 40 mg/kg of PN) and PN group (40 mg/kg).
Results |
PS-MPs intoxication substantially lessened the activities of glutathione peroxidase (GPx), glutathione reductase (GSR), superoxide dismutase (SOD) along with catalase (CAT) while, raised the level of malondialdehyde (MDA) as well as reactive oxygen species (ROS). Additionally, PS-MPs reduced luteinizing hormone (LH), plasma testosterone, follicle-stimulating hormone (FSH) concentration, sperm motility, sperm count, expression of steroidogenic enzymes and Bcl-2 (anti-apoptotic protein) along with the count of spermatogenic cells. While, dead sperm count, sperm abnormalities (tail, neck and head), Bax and caspase-3 (apoptotic proteins) expression along with histopathological anomalies were elevated. Moreover, PS-MPs exposure increased the level of inflammatory markers. However, PN treatment considerably decreased oxidative stress (OS) by reducing ROS as well as increased sperm motility and alleviated all the damages induced by the PS-MPs.
Conclusion |
Therefore, it is concluded that PN may prove a potential therapeutic candidate to restore all the PS-MPs-induced testicular toxicities.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | PS-MPs exposure damaged biochemical, spermatogenic and histological profiles of male albino rats. |
• | PN escalated the activities of anti-oxidant enzymes and decreased MDA as well as ROS levels in male albino rats. |
• | PN supplementation restored the levels of inflammatory and apoptotic markers in male albino rats. |
• | PN increased the expression of steroidogenic enzymes and hormone levels in male albino rats. |
• | Furthermore, PN also improved all the histopathological alterations in the testicular tissues of male albino rats. |
Keywords : Polystyrene Microplastics, Testicular toxicity, Pinostrobin, Oxidative stress, Male reproductive system, Antioxidant
Plan
Vol 162
Article 114686- juin 2023 Retour au numéro
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