Single-cell RNA sequencing in skeletal muscle developmental biology - 29/04/23
Abstract |
Skeletal muscle is the most extensive tissue in mammals, and they perform several functions; it is derived from paraxial mesodermal somites and undergoes hyperplasia and hypertrophy to form multinucleated, contractile, and functional muscle fibers. Skeletal muscle is a complex heterogeneous tissue composed of various cell types that establish communication strategies to exchange biological information; therefore, characterizing the cellular heterogeneity and transcriptional signatures of skeletal muscle is central to understanding its ontogeny’s details. Studies of skeletal myogenesis have focused primarily on myogenic cells' proliferation, differentiation, migration, and fusion and ignored the intricate network of cells with specific biological functions. The rapid development of single-cell sequencing technology has recently enabled the exploration of skeletal muscle cell types and molecular events during development. This review summarizes the progress in single-cell RNA sequencing and its applications in skeletal myogenesis, which will provide insights into skeletal muscle pathophysiology.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Overview and recent advancements of scRNA-seq technologies. |
• | Application of scRNA-seq in skeletal myogenesis. |
• | Prospects of scRNA-seq technologies in the field of skeletal muscle. |
Keywords : ScRNA-seq, Skeletal muscle, Cellular heterogeneity, Pseudotime patterns, Cell–cell communication
Abbreviations : scRNA-seq, LCM, FACS, MACS, UMI, tSNE, UMAP, MTJ, NMJ, MuSC
Plan
Vol 162
Article 114631- juin 2023 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?