Relationship between interferon-induced transmembrane protein 3 and matrix metalloproteinase-9 gene polymorphisms in patients with hepatocellular carcinoma - 27/04/23
, Alyaa R. Elsergany b, Maivel H. Ghattas c, Nancy Mahsoub d, Rania M. Bondok aHighlights |
• | The key molecular biology progression in hepatocellular carcinoma and the relationship between hepatocellular cancer and genetic polymorphisms of IFITM3 and MMP-9. |
• | The TT genotype of MMP-9-1562 C/T and the CC genotype of IFITM3 rs-12252-CC polymorphisms might be associated with the risk of hepatocellular carcinoma. |
• | Screening genotypes of MMP-9 and IFITM3 may help for diagnosis and starting intervention of the disease is necessary. |
Abstract |
Background |
Hepatocellular carcinoma originates from hepatocytes as a result of the effects of numerous genetic variations. Interferon-Induced Transmembrane protein 3 (IFITM3) is involved in the processes of cellular differentiation, apoptosis, cell adhesion, and immune cell regulation. Matrix Metalloproteinase-9 (MMP-9) are zinc dependent endopeptidases that cleave extracellular matrix contents and play an important role in the progression of cancer.
Objective |
The study aimed to outline the key molecular biology progression in hepatocellular carcinoma and the relationship between hepatocellular cancer and genetic polymorphisms of IFITM3 and MMP-9.
Methods |
In total 200 patients with hepatocellular carcinoma patients (n=100) and a control group with Hepatitis C virus (n=100) which collected randomly from the EL-Mansoura oncology center during the interval between June 2020 and October 2021. The expression of MMP-9 and the IFITM3 SNP was investigated. MMP-9 gene polymorphisms were estimated by using PCR-RFLP and IFITM3 gene was detected using DNA sequencing, ELISA was used to measure protein levels of MMP-9 and IFITM3.
Results |
The T allele of MMP-9 was more frequent among patients (n=121) than control subjects (n=71). The C allele of IFITM3 was more frequent among patients (n=112) than control subjects (n=83), polymorphisms of the genes linked to a high risk of disease development, patients of MMP-9 (TT genotype), odd ratio (OR) = 2.63, IFITM3 (CC genotype), OR= 2.43.
Conclusions |
We found that the genetic polymorphisms of MMP-9 and IFITM3 are related to the occurrence and development of hepatocellular carcinoma. This study might be utilized in clinical diagnosis and therapy and to provide a baseline for prevention.
Le texte complet de cet article est disponible en PDF.Keywords : Hepatocellular carcinoma, Hepatitis C virus, Interferon-induced transmembrane protein-3, Matrix metalloproteinase-9, Gene polymorphisms
Abbreviations : HCC, IFITM3, MMP-9, PCR-RFLP, ELISA, HWE, EDTA
Plan
Vol 47 - N° 5
Article 102110- mai 2023 Retour au numéro
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