Chrysin protects against cerebral ischemia-reperfusion injury in hippocampus via restraining oxidative stress and transition elements - 28/03/23
, Jiakang Jiao a, 1 , Mingxue Yan a, 1 , Jingyi Wang a , Qiannan Li a , Lizha Shabuerjiang a , Yinghui Lu a , Qi Song a , Lei Bi a , Guijinfeng Huang a , Xiaolu Zhang a , Yinlian Wen a , Yiran Cui b, ⁎ , Kai Wu a , Gongyu Li a , Peng Wang c , Xin Liu a, ⁎ Abstract |
Chrysin is a natural flavonoid compound that has antioxidant and neuroprotective effects. Cerebral ischemia reperfusion (CIR) is closely connected with increased oxidative stress in the hippocampal CA1 region and homeostasis disorder of transition elements such as iron (Fe), copper (Cu) and zinc (Zn). This exploration was conducted to elucidate the antioxidant and neuroprotective effects of chrysin based on transient middle cerebral artery occlusion (tMCAO) in rats. Experimentally, sham group, model group, chrysin (50.0 mg/kg) group, Ginaton (21.6 mg/kg) group, Dimethyloxallyl Glycine (DMOG, 20.0 mg/kg) + chrysin group and DMOG group were devised. The rats in each group were performed to behavioral evaluation, histological staining, biochemical kit detection, and molecular biological detection. The results indicated that chrysin restrained oxidative stress and the rise of transition element levels, and regulated transition element transporter levels in tMCAO rats. DMOG activated hypoxia-inducible factor-1 subunit alpha (HIF-1α), reversed the antioxidant and neuroprotective effects of chrysin, and increased transition element levels. In a word, our findings emphasize that chrysin plays a critical role in protecting CIR injury via inhibiting HIF-1α against enhancive oxidative stress and raised transition metal levels.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Chrysin reduces CIR-induced pathological damage in rat model of tMCAO. |
• | The antioxidant pharmacological effect of chrysin is mediated through HIF-1α. |
• | The protective effect of chrysin is related to inhibition of Fe, Cu, and Zn overload. |
• | CA1 region of hippocampus is vulnerable to CIR damage. |
• | Chrysin is a potent antioxidant with potential in the treatment of ischemic stroke. |
Abbreviations : ACSL4, ATP7A, BBB, BDNF, CBF, CCA, CIR, COMMD1, COX2, CP, Cu, DMOG, ECA, Fe, GAPDH, GPX4, HE, HIF-1α, ICA, IHC, MDA, MMP2, MMP9, MT3, NGF, NRF2, ROS, RT-qPCR, SLC30A3, SLC7A11, SOD, TEM, TF, TFR1, tMCAO, TTC, WB, Zn
Keywords : Chrysin, Cerebral ischemia-reperfusion (CIR), Hippocampus, Oxidative stress, Transition element, In vivo
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Vol 161
Article 114534- mai 2023 Retour au numéro
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