Depletion of gut microbiota resistance in 5×FAD mice enhances the therapeutic effect of mesenchymal stem cell-derived exosomes - 28/03/23
, Jinlan Jiang a, ⁎ Abstract |
Mesenchymal stem cell-derived exosomes (MSCs-exo) can be used for treating Alzheimer’s disease (AD) by promoting amyloid-β (Aβ) degradation, modulating immune responses, protecting neurology, promoting axonal growth, and improving cognitive impairment. Increasing evidence suggests that the alteration of gut microbiota is closely related to the occurrence and development of Alzheimer's disease. In this study, we hypothesized that dysbiosis of gut microbiota might limit the therapy of MSCs-exo, and the application of antibiotics would improve the therapy.
Methods |
In this original research study, we used MSCs-exo to treat 5 ×FAD mice and fed them antibiotic cocktails for 1 week to detect cognitive ability and neuropathy. The mice’s feces were collected to investigate alterations in the microbiota and metabolites.
Results |
The results revealed that the AD gut microbiota eliminated the therapeutic effect of MSCs-exo, whereas antibiotic modulation of disordered gut microbiota and associated metabolites enhanced the therapeutic effect of MSCs-exo.
Conclusions |
These results encourage the research of novel therapeutics to enhance MSCs-exo treatment for AD, which could benefit a broader range of patients with AD.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | We discovered differences in the gut microbiota and their metabolites between 5 ×FAD and WT mice. |
• | The Alzheimer’s disease gut microbiota eliminated the therapeutic effect of MSCs-exo. |
• | Antibiotic modulation of disordered gut microbiota and associated metabolites enhanced the therapeutic effect of MSCs-exo. |
• | Our findings will encourage the research of novel therapeutics to enhance MSCs-exo treatment for AD. |
Abbreviations : Aβ, Abx, AD, CNS, CLSM, CS, ELISA, EVs, CFC, MWM, GFAP, HE, MSCs, MSCs-exo, NTA, PBS, 16S rRNA, WT
Keywords : Mesenchymal stem cells, Exosomes, Alzheimer’s disease, Gut microbiota, Broad-spectrum antibiotics
Plan
Vol 161
Article 114455- mai 2023 Retour au numéro
Article précédent
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