QiShen YiQi and its components attenuate acute thromboembolic stroke and carotid thrombosis by inhibition of CD62P/PSGL-1-mediated platelet-leukocyte aggregate formation - 26/02/23
Abstract |
Background |
QiShen YiQi (QSYQ) dropping pill, a component-based Chinese medicine consisting of benefiting Qi (YQ) and activating blood (HX) components, has been reported to exert a beneficial effect on cerebral ischemia-induced stroke. However, its efficacy and pharmacological mechanism on acute thromboembolic stroke is not clear.
Purpose |
This study is to explore the preventative effect and pharmacological mechanism of QSYQ and its YQ/HX components on the formation of platelet-leukocyte aggregation (PLA) in acute thromboembolic stroke.
Study design and methods |
In vivo thromboembolic stroke model and FeCl3-induced carotid arterial occlusion models were used. Immunohistochemistry, Western blot, RT-qPCR, and flow cytometry experiments were performed to reveal the pharmacological mechanisms of QSYQ and its YQ/HX components.
Results |
In thromboembolic stroke rats, QSYQ significantly attenuated infarct area, improved neurological recovery, reduced PLA formation, and inhibited P-selection (CD62P)/ P-selectin glycoprotein ligand-1 (PSGL-1) expressions. The YQ component preferentially down-regulated PSGL-1 expression in leukocyte, while the HX component preferentially down-regulated CD62P expression in platelet. In carotid arterial thrombosis mice, QSYQ and its YQ/HX components inhibited thrombus formation, prolonged vessel occlusion time, reduced circulating leukocytes and P-selectin expression. PLA formation and platelet/leukocyte adhesion to endothelial cell were also inhibited by QSYQ and its YQ/HX components in vitro.
Conclusion |
QSYQ and YQ/HX components attenuated thromboembolic stroke and carotid thrombosis by decreasing PLA formation via inhibiting CD62P/PSGL-1 expressions. This study shed a new light on the prevention of thromboembolic stroke.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | QiShen YiQi and its Qi-benefiting/blood-activating components attenuated acute thromboembolic stroke and carotid thrombosis. |
• | QSYQ inhibits platelet-leukocyte aggregate formation and platelet/leukocyte adhesion to endothelial cell. |
• | QSYQ inhibits platelet/leukocyte adhesion molecule CD62P/PSGL-1 expressions. |
• | Qi-benefiting and blood-activating components of QSYQ act synergistically to exert the anti-thrombotic effect. |
Abbreviations : PLA, CD62P, PSGL-1, GPⅠbα, MAC-1, TCM, QSYQ, YQ, HX
Keywords : QiShen YiQi, Thromboembolic stroke, Carotid artery thrombosis, Platelet-leukocyte aggregate, Component-based Chinese medicine
Plan
Vol 160
Article 114323- avril 2023 Retour au numéro
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