Isoalantolactone protects against ethanol-induced gastric ulcer via alleviating inflammation through regulation of PI3K-Akt signaling pathway and Th17 cell differentiation - 26/02/23
, Ma Mi c, ⁎ , Qing Xia b, ⁎ Abstract |
Gastric ulcer (GU) is one of the most prevalent digestive system diseases in humans, and it has been linked to inflammation. Previous studies have demonstrated the anti-inflammatory potential of isoalantolactone (IAL), a sesquiterpene lactone isolated from Radix Inulae. However, the pharmacological effects of IAL on GU and its mechanism of action are still unclear. Hence, the present study is aimed to investigate the anti-inflammatory potential of IAL on GU. Firstly, we assessed the effect of IAL on ethanol-induced injury of human gastric epithelial cells and the levels of inflammatory cytokines in cell culture supernatants. Then, the anti-inflammatory effects of IAL were confirmed in vivo using zebrafish inflammation models. Furthermore, the mechanism of IAL against GU was preliminarily discussed through network pharmacology and molecular docking studies. Quantitative real-time PCR assays were also used to confirm the mechanism of IAL action. ALB, EGFR, SRC, HSP90AA1, and CASP3 were found for the first time as the key targets of the IAL anti-GU. PI3K-Akt signaling pathway and Th17 cell differentiation were identified to play a crucial role in the anti-GU effects of IAL. In conclusion, we found that IAL has anti-inflammatory effects both in vitro and in vivo, and showed potential protective effects against ethanol-induced GU.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Isoalantolactone (IAL) treatment could attenuate human gastric epithelial (GES-1) cell injury induced by ethanol. |
• | IAL showed significant anti-inflammatory effects on three zebrafish inflammation models. |
• | ALB, EGFR, SRC, HSP90AA1, and CASP3 were key targets involved in network regulation. |
• | PI3K-Akt signaling pathway and Th17 cell differentiation were identified as playing vital roles. |
Keywords : Isoalantolactone, Gastric ulcer, Anti-inflammatory effect, Network pharmacology, Zebrafish
Plan
Vol 160
Article 114315- avril 2023 Retour au numéro
Article précédent
- Butyrate ameliorates chronic alcoholic central nervous damage by suppressing microglia-mediated neuroinflammation and modulating the microbiome-gut-brain axis
- Huiling Wei, Chunyang Yu, Chun Zhang, Yi Ren, Li Guo, Ting Wang, Feifei Chen, Yiwei Li, Xiaoxia Zhang, Hao Wang, Juan Liu
- Bias-force guided simulations combined with experimental validations towards GPR17 modulators identification
- Sana Kari, Akshaya Murugesan, Ramesh Thiyagarajan, Srivatsan Kidambi, Jamoliddin Razzokov, Chandrabose Selvaraj, Meenakshisundaram Kandhavelu, Parthiban Marimuthu
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?