Resveratrol alleviated neuroinflammation induced by pseudorabies virus infection through regulating microglial M1/M2 polarization - 26/02/23
, Zhongqiong Yin a, ⁎ Abstract |
Background |
Pseudorabies virus (PRV) infections in susceptible non-porcine species trigger uncontrolled inflammations and eventually fatal encephalitis. Resveratrol (Res) has broad pharmacological functions including anti-virus, anti-inflammation, and neuroprotective.
Purpose |
We attempted to investigate the potential of Res in ameliorating PRV infection pathology in mice and decipher the mechanism of Res in treating PRV.
Methods |
The mice were infected by PRV to investigate the protective effect of Res. Blood-brain barrier (BBB) permeability, H&E/Nissl/TUNEL staining, Real-time PCR and ELISA analyses were performed. Primary microglia and neuron were isolated from mice and cultured. The co-culture model of microglia and neuron was established by transwell. Immunofluorescence assay and flow cytometry were used.
Results |
In this study, we showed that Res ameliorated brain damage by reducing BBB permeability in PRV-infected mice, and diminished the expressions of MMP-2, MMP-9 and ZO-1 in the cortex. Pathological changes of neurons by H&E/Nissl/TUNEL staining suggested that Res could alleviate neuronal lesions. Moreover, Res inhibited the expressions of pro-inflammatory factors (IL-6, TNF-α) and chemokines (CCL3, CXCL10, MCP-1), but increased the expressions of anti-inflammatory factors (IL-4, IL-10) and neurotrophic factor (TGF-β, NGF and GDNF) in brain. In vitro cultured microglia cells, Res could suppress M1 microglia polarization and activate M2 microglia polarization. Co-culture of PRV-infected microglia with neuron cells by transwell system indicated that Res alleviated inflammatory response and neuronal apoptosis.
Conclusion |
This study provided evidence that Res could protect mice from PRV-induced encephalitis through regulation of microglia polarization and neuronal apoptosis suggesting the potential for treatment of viral encephalitis.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Schematic model of the mechanism by which resveratrol suppress PRV infection neuroinflammation by regulating microglia polarization.
Schematic model of the mechanism by which resveratrol suppress PRV infection neuroinflammation by regulating microglia polarization.ga1Le texte complet de cet article est disponible en PDF.
Highlights |
• | Resveratrol ameliorated brain damage and inhibited inflammatory response following PRV infection. |
• | Resveratrol regulates microglial M2 polarization and reduces neuronal apoptosis after PRV infection. |
• | Promising potential of resveratrol in the therapeutic treatment of PRV infection. |
Abbreviations : ACV, Arg-1, BBB, BSA, CCK-8, CCL3, CD206, CD86, CNS, CXCL10, DAPI, DMEM, GDNF, H&E, IL-4, IL-6, IL-10, iNOS, MCP-1, MMP-2, MMP-9, NGF, PI, PNS, Res, SCMC-PSB, TGF-β, TNF-α, ZO-1, Ym1
Keywords : Resveratrol, Pseudorabies virus, Neuroinflammation, Microglial polarization
Plan
Vol 160
Article 114271- avril 2023 Retour au numéro
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