Brain targeted borneol-baicalin liposome improves blood-brain barrier integrity after cerebral ischemia-reperfusion injury via inhibiting HIF-1α/VEGF/eNOS/NO signal pathway - 26/02/23
, Songyu Liu a, 1 , Jinyan Wan a , Yulu Zhang a , Dan Li a , Shuang Yu a , Ai Shi a , Nan Li a, ⁎ , Fei He b, ⁎ Abstract |
Baicalin (BA) is widely used in the treatment of cerebral ischemia-reperfusion injury (CIRI). The key to treating encephalopathy is to increase the amounts of drugs entering the brain. Borneol-baicalin liposome (BO-BA-LP) was prepared in previous research based on the characteristics of borneol (BO) in promoting drug brain entry. In this study, the effect of BO-BA-LP on improving blood-brain barrier (BBB) integrity was researched. Results showed BO-BA-LP may increase ability of BA to penetrate the cell membrane in vitro. Pharmacokinetic results showed the BO-BA-LP could increase concentrations of BA in plasma and brain tissues of normal and CIRI mice. Pharmacological results revealed BO-BA-LP could improve the neurological function, brain edema, and histopathology of CIRI mice. Besides, BO-BA-LP could protect BBB by regulating hypoxia inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF)/endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) pathway. The research showed that BO in BO-BA-LP could increase the absorption of BA by increasing BBB permeability, leading to a better therapeutic effect of BO-BA-LP on CIRI mice.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
The effect of BO-BA-LP on BBB permeability was evaluated by in vitro and in vivo research. The research showed that BO in BO-BA-LP could increase the absorption of BA by increasing BBB permeability, leading to a better therapeutic effect of BO-BA-LP on CIRI mice via inhibiting HIF-1α/VEGF/eNOS/NO signal pathway.
The effect of BO-BA-LP on BBB permeability was evaluated by in vitro and in vivo research. The research showed that BO in BO-BA-LP could increase the absorption of BA by increasing BBB permeability, leading to a better therapeutic effect of BO-BA-LP on CIRI mice via inhibiting HIF-1α/VEGF/eNOS/NO signal pathway.ga1Le texte complet de cet article est disponible en PDF.
Highlights |
• | BO in BO-BA-LP could increase the absorption of BA by increasing BBB permeability. |
• | BO-BA-LP had a better therapeutic effect on CIRI mice than BA-LP. |
• | BO-BA-LP might protect BBB by regulating HIF-1α/VEGF/eNOS/NO pathway. |
Abbreviations : AUC, BA, BA-LP, BBB, BO, BO-BA-LP, CCK-8, CIRI, Cmax, DMEM, EB, ELISA, eNOS, FBS, FITC, HBSS, HE, HIF-1α, HPLC, Na-F, NO, OD, PBS, t1/2, VEGF
Keywords : Cerebral ischemia-reperfusion injury, Borneol-baicalin liposome, Blood-brain barrier, Pharmacodynamics, Pharmacokinetics
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Vol 160
Article 114240- avril 2023 Retour au numéro
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