An exploratory analysis of C-X-C motif chemokine ligand 10 as a new biomarker of asymptomatic Leishmania infantum infection in solid-organ transplant recipients - 08/04/22
, Mario Fernández-Ruiz a, Pilar Hernández-Jiménez a, José María Aguado a, Javier Moreno b, 1, Francisco López-Medrano a, 1Highlights |
• | SOT recipients with asymptomatic Leishmania infection produce high levels of CXCL10. |
• | Whole blood assay is a quick and easy tool for screening SOT recipients. |
• | Monitoring SOT recipients in endemic areas is vital to avoid the risk of developing VL. |
Summary |
Objective |
Sensitive and less laborious assays are needed to detect asymptomatic Leishmania among solid organ transplant (SOT) recipients. Using SLA-stimulated plasma from SOT recipients living where an outbreak of Leishmania infantum occurred, we examined potential biomarkers to identify asymptomatic Leishmania infections. Methods: Concentrations of cytokines/chemokines in plasma from whole blood stimulated with specific Leishmania antigen (SLA) were compared against infection status as determined by a currently used cell proliferation assay.
Results |
Twenty-six percent (13/50) of the SOT recipients had a cell proliferation assay (CPA) indicating asymptomatic infection, and showed higher processed plasma gene-symbol-report/ (CXCL10 or IP-10) concentrations than did non-infected subjects (median 2272.0 pg/ml [IQR 1570–2772] vs. 18.2 pg/ml [IQR 1–150.1]; p<0.0001). CXCL10 showed a sensitivity of 93% and a specificity of 95% compared to CPA. In addition, we demonstrated that the number of asymptomatic infections detected using CXCL10, decreased with distance from a park at the center of the mentioned outbreak.
Conclusion |
CXCL10 in plasma from SLA-stimulated blood could be a robust biomarker of asymptomatic L. infantum infection in solid organ transplant recipients.
Le texte complet de cet article est disponible en PDF.Keywords : Solid-organ transplant recipients, Leishmania, Leishmaniasis, Biomarkers, Asymptomatic infection, IP-10, CXCL10
Plan
Vol 84 - N° 4
P. 573-578 - avril 2022 Retour au numéro
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