Effective combined antiretroviral therapy provides partial immune recovery to mycobacterial antigens in vertically infected, BCG-vaccinated youth living with HIV - 05/03/22
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Abstract |
Background |
We assessed the cytokine response by PBMC of youth living with HIV (YLHIV) under combined antiretroviral therapy (cART) to Mycobacterium tuberculosis (Mtb) and Mycobacterium bovis (BCG) antigens.
Methods |
PBMC from 20 Brazilian YLHIV under cART with long-term (≥1 year) virological control, and 20 healthy controls were cultured for 24–96 h under stimulation with BCG, Mtb lysates, ESAT-6 and SEB. We measured TNF-α, IFN-γ, IL-2, IL-4, IL-5, IL-10 and IL-17 in culture supernatants using a cytometric bead array.
Results |
Controls had higher IFN-γ production at 24, 48, 72 and 96 h upon stimulation with BCG lysate, plateauing at 48 h (Median = 1991 vs. 733 pg/mL; p = 0.01), and after 48–72 h of stimulation with Mtb lysate, plateauing at 48 h (3838 vs. 2069 pg/mL; p = 0.049). YLHIV had higher TNF-α production at all time points upon stimulation with ESAT-6, with highest concentration at 36 h (388 vs. 145 pg/mL; p = 0.02). Within the YLHIV group, total CD4 T cell count and CD4/CD8 ratio were associated with IFN-γ response to Mtb lysate and ESAT-6, respectively.
Conclusions |
Even under long-term cART, YLHIV seem to have a suboptimal T-helper-1 response to mycobacterial antigens. This can be explained by early immunodeficiency in vertical infection, with lasting damage.
Le texte complet de cet article est disponible en PDF.Keywords : Mycobacterium tuberculosis, HIV, Cytokine, Young adult, BCG vaccine, Antiretroviral therapy, Highly active
Abbreviations : PBMC, BCG, ESAT-6, SEB, TNF-α, IFN-γ, IL
Plan
☆ | Presented in part at the Keystone Symposia Conference “New Developments in Our Basic Understanding of Tuberculosis”, January 14–17, 2017, Vancouver, British Columbia, Canada (Poster #3034). |
Vol 133
Article 102170- mars 2022 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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