Effective combined antiretroviral therapy provides partial immune recovery to mycobacterial antigens in vertically infected, BCG-vaccinated youth living with HIV - 05/03/22
Abstract |
Background |
We assessed the cytokine response by PBMC of youth living with HIV (YLHIV) under combined antiretroviral therapy (cART) to Mycobacterium tuberculosis (Mtb) and Mycobacterium bovis (BCG) antigens.
Methods |
PBMC from 20 Brazilian YLHIV under cART with long-term (≥1 year) virological control, and 20 healthy controls were cultured for 24–96 h under stimulation with BCG, Mtb lysates, ESAT-6 and SEB. We measured TNF-α, IFN-γ, IL-2, IL-4, IL-5, IL-10 and IL-17 in culture supernatants using a cytometric bead array.
Results |
Controls had higher IFN-γ production at 24, 48, 72 and 96 h upon stimulation with BCG lysate, plateauing at 48 h (Median = 1991 vs. 733 pg/mL; p = 0.01), and after 48–72 h of stimulation with Mtb lysate, plateauing at 48 h (3838 vs. 2069 pg/mL; p = 0.049). YLHIV had higher TNF-α production at all time points upon stimulation with ESAT-6, with highest concentration at 36 h (388 vs. 145 pg/mL; p = 0.02). Within the YLHIV group, total CD4 T cell count and CD4/CD8 ratio were associated with IFN-γ response to Mtb lysate and ESAT-6, respectively.
Conclusions |
Even under long-term cART, YLHIV seem to have a suboptimal T-helper-1 response to mycobacterial antigens. This can be explained by early immunodeficiency in vertical infection, with lasting damage.
Le texte complet de cet article est disponible en PDF.Keywords : Mycobacterium tuberculosis, HIV, Cytokine, Young adult, BCG vaccine, Antiretroviral therapy, Highly active
Abbreviations : PBMC, BCG, ESAT-6, SEB, TNF-α, IFN-γ, IL
Plan
| ☆ | Presented in part at the Keystone Symposia Conference “New Developments in Our Basic Understanding of Tuberculosis”, January 14–17, 2017, Vancouver, British Columbia, Canada (Poster #3034). |
Vol 133
Article 102170- mars 2022 Retour au numéro
Article précédent
- Characterization of embB mutations involved in ethambutol resistance in multi-drug resistant Mycobacterium tuberculosis isolates in Zambia
- Precious Bwalya, Eddie S. Solo, Joseph Y. Chizimu, Dipti Shrestha, Grace Mbulo, Jeewan Thapa, Chie Nakajima, Yasuhiko Suzuki
- Concurrent evaluation of cytokines improves the accuracy of antibodies against Mycobacterium tuberculosis antigens in the diagnosis of active tuberculosis
- Ruschca Jacobs, Dolapo O. Awoniyi, Ralf Baumann, Kim Stanley, Shirley McAnda, Susanne Kaempfer, Stephanus T. Malherbe, Mahavir Singh, Gerhard Walzl, Novel N. Chegou, the AE-TBC Consortium, Gerhard Walzl, Novel N. Chegou, Magdalena Kriel, Gian van der Spuy, Andre G. Loxton, Kim Stanley, Stephanus T. Malherbe, Belinda Kriel, Jayne S. Sutherland, Olumuyiwa Owolabi, Abdou Sillah, Joseph Mendy, Awa Gindeh, Simon Donkor, Toyin Togun, Martin Ota, Amelia C. Crampin, Felanji Simukonda, Alemayehu Amberbir, Femia Chilongo, Rein Houben, Desta Kassa, Atsbeha Gebrezgeabher, Getnet Mesfin, Yohannes Belay, Gebremedhin Gebremichael, Yodit Alemayehu, Marieta van der Vyver, Faustina N. Amutenya, Josefina N. Nelongo, Lidia Monye, Jacob A. Sheehama, Scholastica Iipinge, Harriet Mayanja-Kizza, Anna Ritah Namuganga, Grace Muzanye, Mary Nsereko, Pierre Peters, Rawleigh Howe, Adane Mihret, Yonas Bekele, Bamlak Tessema, Lawrence Yamuah, Tom H.M. Ottenhoff, Annemieke Geluk, Kees Franken, Jolien J. van der Ploeg-van Schip, Paul L.A.M. Corstjens, Elisa M. Tjon Kon Fat, Claudia J. de Dood, Ida Rosenkrands, Claus Aagaard, Stefan H.E. Kaufmann, Maria M. Esterhuyse, Jacqueline M. Cliff, Hazel M. Dockrell
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?