Nasopharyngeal Microbiota as an early severity biomarker in COVID-19 hospitalised patients - 02/03/22

Doi : 10.1016/j.jinf.2021.12.030

Maria Paz Ventero ^a,^{^{1
These authors contributed to the manuscript equally and share the first authorship}}, Oscar Moreno-Perez ^b,^c,^{^{1
These authors contributed to the manuscript equally and share the first authorship}}, Carmen Molina-Pardines ^a,^⁎ , Andreu Paytuví-Gallart ^d,^e, Vicente Boix ^f,^c, Isabel Escribano ^a, Irene Galan ^g, Pilar González-delaAleja ^f, Mario López-Pérez ^h,^a, Rosario Sánchez-Martínez ⁱ, Esperanza Merino ^f,^{^{2
Esperanza Merino and Juan Carlos Rodríguez are Joint Senior Authors}}, Juan Carlos Rodríguez ^a,^h,^{^{2
Esperanza Merino and Juan Carlos Rodríguez are Joint Senior Authors}}
^a Microbiology Service, Alicante General University Hospital - Alicante Institute of Health and Biomedical Research (ISABIAL), Alicante, Spain
^b Endocrinology and Nutrition department, Alicante General University Hospital - Alicante Institute of Health and Biomedical Research (ISABIAL), Alicante, Spain
^c Clinical Medicine department, Miguel Hernández University, Elche, Spain
^d Sequentia Biotech, Carrer Comte d'Urgell 240, 08036 Barcelona, Spain
^e Open University of Catalonia (UOC), Rambla del Poblenou, 156, 08018 Barcelona, Spain
^f Unit of Infectious Diseases, Alicante General University Hospital - Alicante Institute of Health and Biomedical Research (ISABIAL), Alicante, Spain
^g Pneumology department, Alicante General University Hospital - Alicante Institute of Health and Biomedical Research (ISABIAL), Alicante, Spain
^h Evolutionary Genomics Group, División de Microbiología, Universidad Miguel Hernández, Spain
ⁱ Internal Medicine department, Alicante General University Hospital - Alicante Institute of Sanitary and Biomedical Research (ISABIAL), Alicante, Spain

^⁎Corresponding author.

connectez-vous ou créez un compte

Bienvenue sur EM-consulte, la référence des professionnels de santé.
Article gratuit.

Connectez-vous pour en bénéficier!

Highlights

•	Lower microbiota diversity is linked to a worse prognosis, and increased likelihood of a fatal outcome.
•	The presence of Selenomonas SPP., Filifactor SPP., Actinobacillus SPP. or Chroococcidiopsis SPP., is associated with a reduced risk of invasive mechanical ventilation being required.
•	The microbiome could be used as a prognostic factor.

Le texte complet de cet article est disponible en PDF.

Summary

This study aimed to analyse the diversity and taxonomic composition of the nasopharyngeal microbiota, to determine its association with COVID-19 clinical outcome. To study the microbiota, we utilized 16S rRNA sequencing of 177 samples that came from a retrospective cohort of COVID-19 hospitalized patients. Raw sequences were processed by QIIME2. The associations between microbiota, invasive mechanical ventilation (IMV), and all-cause mortality were analysed by multiple logistic regression, adjusted for age, gender, and comorbidity. The microbiota α diversity indexes were lower in patients with a fatal outcome, whereas the β diversity analysis showed a significant clustering in these patients. After multivariate adjustment, the presence of Selenomonas spp., Filifactor spp., Actinobacillus spp., or Chroococcidiopsis spp., was associated with a reduction of more than 90% of IMV. Higher diversity and the presence of certain genera in the nasopharyngeal microbiota seem to be early biomarkers of a favourable clinical evolution in hospitalized COVID-19 patients.

Le texte complet de cet article est disponible en PDF.

Graphical abstract

Image, graphical abstract.

Le texte complet de cet article est disponible en PDF.

Keywords : Microbiota, COVID-19, SARS-COV-2, Severity, Biomarker, Prognosis

Plan

Introduction

Materials and methods

Patients and study design and setting

Variables and data collection

DNA isolation and microbiota amplicon next-generation sequencing (NGS)

Bioinformatic analyses

Statistical analysis

Ethics statement and data availability

Results

Participans and descriptive data

Diversity analysis and outcomes

Taxonomic analysis and outcomes

Differently represented genera and outcomes

Discussion

Funding

Export

Vol 84 - N° 3

P. 329-336 - mars 2022 Retour au numéro

Article précédent

Maternal BCG primes for enhanced health benefits in the newborn
Frederik Schaltz-Buchholzer, Christian Bjerregård Øland, Mike Berendsen, Morten Bjerregaard-Andersen, Elise Brenno Stjernholm, Christian N Golding, Ivan Monteiro, Peter Aaby, Christine Stabell Benn

| Article suivant

Clostridium innocuum infection in hospitalised patients with inflammatory bowel disease
Puo-Hsien Le, Cheng-Tang Chiu, Pai-Jui Yeh, Yu-Bin Pan, Cheng-Hsun Chiu

Bienvenue sur EM-consulte, la référence des professionnels de santé.

connectez-vous ou créez un compte

Nasopharyngeal Microbiota as an early severity biomarker in COVID-19 hospitalised patients - 02/03/22

Highlights

Summary

Graphical abstract

Plan

Export citations

Fichier

Contenu

Accès rapides

Mon compte

Aide & support

Plateformes Elsevier Masson

Déclaration CNIL