Ascaris lumbricoides and ticks associated with sensitization to galactose α1,3-galactose and elicitation of the alpha-gal syndrome - 03/02/22
Abstract |
Background |
IgE to galactose alpha-1,3 galactose (alpha-gal) causes alpha-gal syndrome (delayed anaphylaxis after ingestion of mammalian meat). Development of sensitization has been attributed to tick bites; however, the possible role of other parasites has not been well studied.
Objective |
Our aims were to assess the presence, relative abundances, and site of localization of alpha-gal–containing proteins in common ectoparasites and endoparasites endemic in an area of high prevalence of alpha-gal syndrome, as well as to investigate the ability of ascaris antigens to elicit a reaction in a humanized rat basophil in vitro sensitization model.
Methods |
Levels of total IgE, Ascaris-specific IgE, and alpha-gal IgE were measured in sera from patients with challenge-proven alpha-gal syndrome and from controls without allergy. The presence, concentration, and localization of alpha-gal in parasites were assessed by ELISA, Western blotting, and immunohistochemistry. The ability of Ascaris lumbricoides antigen to elicit IgE-dependent reactivity was demonstrated by using the RS-ATL8 basophil reporter system.
Results |
Alpha-gal IgE level correlated with A lumbricoides–specific IgE level. Alpha-gal protein at 70 to 130 kDa was detected in A lumbricoides at concentrations higher than those found in Rhipicephalus evertsi and Amblyomma hebraeum ticks. Immunohistochemistry was used to localize alpha-gal in tick salivary acini and the helminth gut. Non–alpha-gal–containing A lumbricoides antigens activated RS-ATL8 basophils primed with serum from subjects with alpha-gal syndrome.
Conclusion |
We demonstrated the presence, relative abundances, and site of localization of alpha-gal–containing proteins in parasites. The activation of RS-ATL8 IgE reporter cells primed with serum from subjects with alpha-gal syndrome on exposure to non–alpha-gal–containing A lumbricoides proteins indicates a possible role of exposure to A lumbricoides in alpha-gal sensitization and clinical reactivity.
Le texte complet de cet article est disponible en PDF.Key words : Alpha-gal, food allergy, galactose-alpha-1,3-galactose, helminths, meat allergy, red meat allergy, ticks, Ascaris lumbricoides, anaphylaxis, Rhipicephalus evertsi, Amblyomma hebraeum
Abbreviations used : Alpha-gal, Asc l, E/S, BTG, GSI-B4, HRP, PBS-T, scFv, Strep-HRP
Plan
| Supported by the Birmingham-Nottingham Strategic Collaboration Fund (to W.G.C.H. and F.H.F.); the LOEWE Centre DRUID within the Hessian Excellence Initiative (P.P., B.P.M., and F.H.F.), and a National Research Foundation Innovation M&D Scholarship (to T.M.). |
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| Disclosure of potential conflicts of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 149 - N° 2
P. 698 - février 2022 Retour au numéro
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