IL-17A–producing sinonasal MAIT cells in patients with chronic rhinosinusitis with nasal polyps - 03/02/22

Doi : 10.1016/j.jaci.2021.07.037

Min-Seok Rha, MD, PhD ^a,^b, Young Hoon Yoon, MD, PhD ^c, June-Young Koh, MD ^a, Jae Hyung Jung, BSc ^a, Ha Seok Lee, MD ^a, Soo Kyoung Park, MD, PhD ^c, Su-Hyung Park, PhD ^a, Yong Min Kim, MD, PhD ^c,^d,^⁎ , Ki-Sang Rha, MD, PhD ^c,^⁎ , Eui-Cheol Shin, MD, PhD ^a,^⁎
^a Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea
^b Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea
^c Department of Otorhinolaryngology–Head and Neck Surgery, Research Institute for Medical Science, Chungnam National University School of Medicine, Daejeon, Korea
^d Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Korea

^∗Corresponding author: Eui-Cheol Shin, MD, PhD, Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.Laboratory of Immunology and Infectious DiseasesGraduate School of Medical Science and EngineeringKAIST291 Daehak-roYuseong-guDaejeon34141Republic of Korea^∗∗Ki-Sang Rha, MD, PhD, Department of Otorhinolaryngology–Head and Neck Surgery, Research Institute for Medical Science, Chungnam National University School of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon 35015, Republic of Korea.Department of Otorhinolaryngology–Head and Neck SurgeryResearch Institute for Medical ScienceChungnam National University School of Medicine282 Munhwa-roJung-guDaejeon35015Republic of Korea^∗∗∗Yong Min Kim, MD, PhD, Department of Otorhinolaryngology–Head and Neck Surgery, Research Institute for Medical Science, Chungnam National University School of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon 35015, Republic of Korea.Department of Otorhinolaryngology–Head and Neck SurgeryResearch Institute for Medical ScienceChungnam National University School of Medicine282 Munhwa-roJung-guDaejeon35015Republic of Korea

Abstract

Background

Diverse immune cells contribute to the pathogenesis of chronic rhinosinusitis (CRS), an inflammatory disease of the nasal cavity and paranasal sinuses. However, whether mucosal-associated invariant T (MAIT) cells are present in human sinonasal tissues remains unclear. Furthermore, the characteristics of sinonasal MAIT cells have not been studied in patients with CRS.

Objective

We investigated the phenotype, function, and clinical implications of MAIT cells in patients with CRS.

Methods

Peripheral blood and sinonasal tissue were obtained from patients with CRS with (CRSwNP) or without nasal polyps (CRSsNP) and healthy controls. MAIT cells were analyzed by flow cytometry.

Results

We found that MAIT cells are present in human sinonasal tissues from healthy controls and patients with CRS. The sinonasal MAIT cell population, but not peripheral blood MAIT cells, from patients with CRSsNP, noneosinophilic CRSwNP (NE-NP), or eosinophilic CRSwNP (E-NP) had a significantly higher frequency of activated cells marked by CD38 expression. In functional analysis, the sinonasal MAIT cell population from NE-NP and E-NP had a significantly higher frequency of IL-17A⁺ cells but lower frequency of IFN-γ⁺ or TNF⁺ cells than control sinonasal tissues. Furthermore, CD38 expression and IL-17A production by sinonasal MAIT cells significantly correlated with disease extent evaluated by the Lund-Mackay computed tomography score in patients with E-NP.

Conclusions

Sinonasal MAIT cells exhibit an activated phenotype and produce higher levels of IL-17A in patients with CRSwNP. These alterations are associated with the extent of disease in patients with E-NP.

Le texte complet de cet article est disponible en PDF.

Graphical abstract

Le texte complet de cet article est disponible en PDF.

Key words : Chronic rhinosinusitis, nasal polyp, mucosal-associated invariant T cells, MAIT cells, IL-17A

Abbreviations used : 5-OP-RU, CRS, CRSsNP, CRSwNP, CT, E-NP, ILC2, MAIT, MR1, NE-NP, NP, NSAID, PB, PMA, TCR, UP

Plan

Methods

Study subjects

Cell isolation

Multicolor flow cytometry

In vitro stimulation for intracellular cytokine staining

Statistical analysis

Results

MAIT cells are present in human sinonasal tissues

MAIT cells with a tissue-resident phenotype are enriched in sinonasal tissue

Sinonasal MAIT cells exhibit an activated phenotype in patients with CRS

IL-17A–producing sinonasal MAIT cells are increased in patients with CRSwNP

IL-17A production by sinonasal MAIT cells correlates with disease extent in patients with E-NP

Discussion

	This work was supported by the National Research Foundation, Republic of Korea, Grant (NRF-2018M3A9D3079498), which is funded by the Ministry of Science and ICT (MSIT).
	Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest.

Export

Vol 149 - N° 2

P. 599 - février 2022 Retour au numéro

Article précédent

Neuroimaging and biomarker evidence of neurodegeneration in asthma
Melissa A. Rosenkranz, Douglas C. Dean, Barbara B. Bendlin, Nizar N. Jarjour, Stephane Esnault, Henrik Zetterberg, Amanda Heslegrave, Michael D. Evans, Richard J. Davidson, William W. Busse

| Article suivant

Extrafollicular PD-1^highCXCR5^–CD4⁺ T cells participate in local immunoglobulin production in nasal polyps
Zhi-Chao Wang, Yin Yao, Cai-Ling Chen, Cui-Lian Guo, Hong-Xia Ding, Jia Song, Zhe-Zheng Wang, Nan Wang, Xue-Li Li, Bo Liao, Yang Yang, Di Yu, Zheng Liu

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

Déjà abonné à cette revue ?

connectez-vous ou créez un compte

IL-17A–producing sinonasal MAIT cells in patients with chronic rhinosinusitis with nasal polyps - 03/02/22

Abstract

Background

Objective

Methods

Results

Conclusions

Graphical abstract

Plan

Export citations

Fichier

Contenu

Accès rapides

Mon compte

Aide & support

Plateformes Elsevier Masson

Déclaration CNIL