Evaluation of a new assay for nontuberculous mycobacteria species identification in diagnostic material and cultures - 23/09/21
, Vera Ustinova a, Sofya Andreevskaya a, Elena Larionova a, Ekaterina Kiseleva a, Larisa Chernousova a, Dmitry Varlamov b, Dmitry Sochivko b, Atadzhan Ergeshov aAbstract |
The purpose of the present study was to evaluate a real-time PCR system for 12 nontuberculous mycobacteria (NTM) species identification developed by Central Tuberculosis Research Institute (CTRI; Moscow, Russia) in cooperation with Syntol LLC (Moscow, Russia). NTM cultures (210 strains, 19 species), Mycobacterium tuberculosis complex (MTBC) cultures (21 strains, 2 species), non-mycobacterial microorganisms (18 strains, 13 species) were used for the first stage of the assay evaluation. Clinical samples (sputum, N = 973) positive for smear microscopy and MTBC/NTM DNA by a PCR-based screening assay collected from 819 patients were used for specificity and sensitivity evaluation. Sensitivity for determining the NTM species directly from diagnostic material was 99.71%, with the specificity of 100%. The sensitivity and specificity for NTM species identification in cultures was 99.67% and 100%, respectively. Both sensitivity and specificity for determining MTBC in cultures was 100%.
Le texte complet de cet article est disponible en PDF.Highlights |
• | The Real-Time PCR assay identifies 12 NTM species and allows to identify other NTM species as members of NTM group |
• | The assay differentiates between NTM and MTBC |
• | The assay allows to use both cultures and diagnostic material for the species identification |
Keywords : Mycobacterium tuberculosis, Nontuberculous mycobacteria, Real-time PCR
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Vol 130
Article 102124- septembre 2021 Retour au numéro
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