Limited literature is available for bevacizumab exposure-response relationship and there is not a concentration threshold associated with an optimal disease control. This prospective observational study in patients with metastatic colorectal cancer (mCRC) aims to evaluate, in a real-life setting, the relationship between bevacizumab through concentrations at steady state (C_{trough, SS}) and disease control. C_{trough, SS} were drawn, coinciding with the radiological evaluation of the response (progression or clinical benefit). Generalized estimating equations (GEE) analysis was performed. To test the association between C_{trough, SS} in each patient with overall survival (OS) or progression-free survival (PFS), Cox proportional hazard models were developed. Data included 50 bevacizumab C_{trough, SS} from 27 patients. The GEE model did not suggest any positive association between bevacizumab C_{trough, SS} and clinical benefit (OR 0.99, 95% CI: 0.98–1.02, p = 0.863). The Cox regression showed association between higher median C_{trough, SS} with better OS (HR 0.86, 95% CI: 0.73–1.01, p = 0.060), but not with PFS. We cannot confirm a relationship between bevacizumab C_{trough, SS} and clinical benefit but this is the first real-world study trying to show a relationship between bevacizumab C_{trough, SS} and disease control in mCRC. It was conducted in a small sample size which reduces the level of evidence. Further controlled randomized studies with a sufficient number of patients are required.