High-fat diet induces a predisposition to follicular hyperkeratosis and neutrophilic folliculitis in mice - 04/08/21
, Tomohito Sato, MD, PhD e, Md. Al Mamun, PhD e, Zachary Chow, PhD b, Kaibo Duan, PhD a, Josephine Lum, BSc a, Kahbing Jasmine Tan, BSc a, b, Kaori Tomari, BSc c, Reiko Sato, MD a, c, Akihiko Kitoh, MD, PhD c, Angeline S.L. Tay, PhD b, John E.A. Common, PhD b, Ng Lai Guan, PhD a, Mitsutoshi Setou, MD, PhD e, Florent Ginhoux, PhD a, b, ⁎ , Kenji Kabashima, MD, PhD a, b, c, ⁎ Abstract |
Background |
Neutrophilic folliculitis is an inflammatory condition of hair follicles. In some neutrophilic folliculitis, such as in patients with acne and hidradenitis suppurativa, follicular hyperkeratosis is also observed. Neutrophilic folliculitis is often induced and/or exacerbated by a high-fat diet (HFD). However, the molecular mechanisms by which an HFD affects neutrophilic folliculitis are not fully understood.
Objective |
Our aim was to elucidate how an HFD promotes the development of neutrophilic folliculitis.
Methods |
Mice were fed an HFD, and their skin was subjected to histologic, RNA sequencing, and imaging mass spectrometry analyses. To examine the effect of an HFD on neutrophil accumulation around the hair follicles, phorbol 12–myristate 13–acetate (PMA) was used as an irritant to the skin.
Results |
Histologic analysis revealed follicular hyperkeratosis in the skin of HFD-fed mice. RNA sequencing analysis showed that genes related to keratinization, especially in upper hair follicular keratinocytes, were significantly upregulated in HFD-fed mice. Application of PMA to the skin induced neutrophilic folliculitis in HFD-fed mice but not in mice fed a normal diet. Accumulation of neutrophils in the skin and around hair follicles was dependent on CXCR2 signaling, and CXCL1 (a CXCR2 ligand) was produced mainly by hair follicular keratinocytes. Imaging mass spectrometry analysis revealed an increase in fatty acids in the skin of HFD-fed mice. Application of these fatty acids to the skin induced follicular hyperkeratosis and caused PMA-induced neutrophilic folliculitis even in mice fed a normal diet.
Conclusion |
An HFD can facilitate the development of neutrophilic folliculitis with the induction of hyperkeratosis of hair follicles and increased neutrophil infiltration around the hair follicles via CXCR2 signaling.
Le texte complet de cet article est disponible en PDF.Key words : High-fat diet, fatty acids, folliculitis, CXCL1, CXCL2, CXCR2, neutrophil, palmitic acid, hair follicle, acne, hidradenitis suppurativa
Abbreviations used : HFD, ND, PKC, PMA, RNA-seq
Plan
| Supported by Singapore Immunology Network and SRIS core funding; the Agency for Science, Technology and Research (grant H17/01/a0/004); the Skin Research Institute of Singapore; grants from the Japan Society for the Promotion of Science (grants JP19K08790 and JP15H05906 [to T.H.] and grant 263395 [to K.K.]), the Takeda Science Foundation [to K.K.], and the Japan Agency for Medical Research and Development (grants 16ek0410011h0003, 16he0902003h0002, and JP19gm1210006 [to K.K.]). S.N. is supported by the Uehara Memorial Foundation. |
|
| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 148 - N° 2
P. 473 - août 2021 Retour au numéro
Article précédent
- Bone morphogenetic protein-2 as a novel biomarker for refractory chronic rhinosinusitis with nasal polyps
- Jin Youp Kim, Suha Lim, Hee-Suk Lim, Yi-Sook Kim, Kyoung Mi Eun, Roza Khalmuratova, Yuju Seo, Joon Kon Kim, Young Seok Kim, Min-Kyung Kim, Siyeon Jin, Seung Cheol Han, Suyeon Pyo, Seung-No Hong, Jong-Wan Park, Hyun-Woo Shin, Dae Woo Kim
- A unique esophageal extracellular matrix proteome alters normal fibroblast function in severe eosinophilic esophagitis
- Lance Y. Hsieh, Austin W.T. Chiang, Loan D. Duong, Chih-Chung Kuo, Stephanie X. Dong, Ranjan Dohil, Richard Kurten, Nathan E. Lewis, Seema S. Aceves
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?