Potential biomarker enhancing the activity of tuberculosis, hsa-miR-346 - 27/07/21
, Kazumi Nishio c, Asami Kohsaka a, Eiko Tamizu a, Yasushi Nakano c, Junko Kagyo c, d, Yukiko Nakajima e, Ryosuke Arai c, Hanako Hasegawa c, Kenichi Arakawa f, Shoko Kashimura a, Ryota Ishii g, h, Naoki Miyazaki g, Yoshifumi Uwamino a, i, Naoki Hasegawa aAbstract |
Objectives |
To determine the usefulness of hsa-miR-346, a potential biomarker enhancing the activity of non-tuberculous mycobacterial diseases, as a biomarker of tuberculosis activity.
Methods |
We investigated whether hsa-miR-346 is secreted by human macrophages infected with Mycobacterium tuberculosis (M. tuberculosis) in an in vitro study. In addition, a cross-sectional study was conducted first to evaluate whether serum hsa-miR-346 is elevated in patients with tuberculosis compared with that in healthy individuals. Second, we conducted a retrospective study to evaluate whether anti-tuberculosis treatment reduces serum hsa-miR-346 levels.
Results |
Log hsa-miR-346 levels were significantly elevated in the supernatant of human macrophages infected with M. tuberculosis in a dose-dependent manner. The mean serum log hsa-miR-346 levels were −15.48 (−15.76 to −15.21) in patients with tuberculosis and −16.12 (−16.29 to −15.95) in healthy volunteers, which significantly differed. In addition, hsa-miR-346 significantly decreased at 2 months from starting an anti-tuberculosis treatment.
Conclusions |
We consider hsa-miR-346 as a potential biomarker enhancing the tuberculosis activity.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Treatment monitoring is essential for the appropriateness of tuberculosis treatment. |
• | Biomarkers that predict tuberculosis treatment outcomes are needed. |
• | We found hsa-miR-346 was secreted from by human macrophages infected with M. tuberculosis. |
• | Serum hsa-miR-346 level declined during the second month of treatment with bacteriological improvement. |
• | We added hsa-miR-346 as a potential biomarker enhancing the tuberculosis activity. |
Keywords : Biomarker, hsa-miR-346, micro RNA, Tuberculosis
Plan
Vol 129
Article 102101- juillet 2021 Retour au numéro
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