Recombinant GII.P16 genotype challenges RT-PCR-based typing in region A of norovirus genome - 19/06/21
, Simona De Grazia aHighlights |
• | In latest years GII.4[P16] and GII.2[P16] noroviruses have become predominant. |
• | GII.P16 strains failed to be typed in polymerase region A with JV12A/JV13B primers. |
• | Modified primer sets were tested to improve typing of the polymerase gene. |
• | The use of degenerate primers increased the typing rate in region A in 2016–2019. |
• | The plasticity of norovirus genomes requires continuous updates of typing primers. |
Abstract |
Objectives |
In latest years GII.4[P16] and GII.2[P16] noroviruses have become predominant in some temporal/geographical settings. In parallel with the emergence of the GII.P16 polymerase type, norovirus surveillance activity in Italy experienced increasing difficulties in generating sequence data on the RNA polymerase genomic region A, using the widely adopted JV12A/JV13B primer set. Two sets of modified primers (Deg1 and Deg2) were tested in order to improve amplification and typing of the polymerase gene.
Methods |
Amplification and typing performance of region A primers was assessed in RT-PCR on 452 GII norovirus positive samples obtained from 2194 stool samples collected in 2016–2019 from children hospitalized with acute gastroenteritis.
Results |
The use of Deg1 increased the rate of samples types in region A from 49.5% to 81.4% and from 21.9% to 69.7% in 2016 and 2017, respectively. The rate of Deg1 typed samples remained high in 2018 (90.1%), but sharply decreased to 11.8% in 2019. The second primers set, Deg2, was able to increase to 64.9% the rate of 2019 samples typed in region A, while typing efficiently 73.2%, 69%, and 86.4% of samples collected in 2016, 2017 and 2018, respectively.
Conclusions |
The plasticity of norovirus genomes requires continuous updates of the primers used for strain characterization.
Le texte complet de cet article est disponible en PDF.Keywords : Norovirus, GII.P16, Typing, Polymerase, Degenerate primers
Plan
Vol 83 - N° 1
P. 69-75 - juillet 2021 Retour au numéro
Article précédent
- Apoptotic caspases suppress Mycobacterium bovis-induced IFN-? production in murine macrophage
- Yinjuan Song, Yuhui Dong, Yi Liao, Zhengmin Liang, Jiao Yao, Xiangmei Zhou
- Molecular surveillance of HIV-1 newly diagnosed infections in Shenzhen, China from 2011 to 2018
- Dong Zhang, Chenli Zheng, Hanping Li, Hao Li, Yongjian Liu, Xiaolin Wang, Lei Jia, Lin Chen, Zhengrong Yang, Yongxia Gan, Yifan Zhong, Jingwan Han, Tianyi Li, Jingyun Li, Jin Zhao, Lin Li
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?