Intranasal or oral Lactobacilli administration: Which one is best for fighting against Pseudomonas aeruginosa (PA) respiratory tract infections? - 09/06/21
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Résumé |
Introduction |
In CF patients, PA chronic lung infections combined with acquisition of antibiotics resistance leads to therapeutic deadlock. Among non-antibiotic alternative, the use of Lactobacilli is promising since its oral administration (OA) stimulates immune system, decreases nosocomial pneumonia & CF exacerbations incidence. IntranasaI administration (IA) stimulates respiratory immunity & modifies lung architecture. Screened from a collection of 50 CF patients expectorations, 3 Lactobacillus (L. paracasei Lp, L. salivarius Ls & L. brevis Lb) respiratory strains were identified for its anti-PA properties in vitro.
Methods |
Selected strains were tested, alone or cocktail (Lpsb, Lsb) for preventive effect in a murine model of acute PA pneumonia. Lactobacillus GG was used as a control. IA & OA of Lactobacillus strains (106CFU/mice) 18h prior to PA infection (106CFU/mice) was evaluated in 12 groups of C57BL/6 mice (Lpsb, Lsb, Lp, Ls, Lb, PA, Lpsb+PA, Lp+PA, Ls+PA, Lb+PA, Lsb+PA, LGG+PA) & 3 groups (Lsb, Lsb+PA, LGG+PA) respectively. At 24h PA post-infection (pi), lung & serum were collected for bacterial counts & cytokines analysis.
Results |
The Lpsb cocktail increased the survival rate to 7 days (100% P<0.001) compared to PA group (11.7%). Lactobacillus treatment decreased the PA lung load 24h pi compared to PA group with a higher effect for Lpsb, Lsb, Ls & Lb group (reduction >1log10 P<0.05). Interestingly, our Lactobacillus strains had better anti-PA properties than the LGG strain & its IA led to a better reduction of PA lung load 24h pi than OA. Preventive Lactobacillus treatment decreased proinflammatory cytokines.
Conclusions |
This study demonstrated the better protective efficacy of live Lactobacillus IA vs OA against murine PA pneumonia. Mechanistic approaches are under progress. L. salivarius & L. brevis are promising beneficial strains in the context of lung infection in CF.
Le texte complet de cet article est disponible en PDF.Keywords : Infection, Inflammation
Plan
Vol 38 - N° 6
P. 583 - juin 2021 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.