Multiphoton microscopy for pre-clinical evaluation of flow-diverter stents for treating aneurysms - 04/05/21
, Jonathan Cortese b, c, Raphaël Blanc d, Charbel Mounayer a, e, Aymeric Rouchaud a, ⁎, e Bienvenue sur EM-consulte, la référence des professionnels de santé.
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| pages | 7 |
| Iconographies | 5 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | Detailed analyses of neo-endothelialisation and ECM evolution after flow-diverter stent (FDS). |
• | Autofluorescence, endothelial cells and second harmonic generation (SHG) signals from biological tissues were used to evaluate the endovascular treatment of intracranial aneurysms. |
• | Improved label-free multiphoton microscopy quantification of FDS integration into the arterial wall. |
Abstract |
Background |
Conventional histological analyses are the gold standard for the study of aneurysms and vascular pathologies in pre-clinical research. Over the past decade, in vivo and ex vivo imaging using multiphoton microscopy have emerged as powerful pre-clinical tools for detailed tissue analyses that can assess morphology, the extracellular matrix (ECM), cell density and vascularisation. Multiphoton microscopy allows for deeper tissue penetration with minor phototoxicity.
Objective |
The present study aimed to demonstrate the current status of multimodality imaging, including multiphoton microscopy, for detailed analyses of neo-endothelialisation and ECM evolution after flow-diverter stent (FDS) treatment in an experimental rabbit model of aneurysms.
Methods |
Multiphoton microscopy tools for assessing autofluorescence and second harmonic generation (SHG) signals from biological tissues were used to evaluate the endovascular treatment of intracranial aneurysms in an animal model of aneurysms (pig, rabbit). Results from multiphoton microscopy were compared to those from standard histology, electronic and bright field microscopy.
Conclusions |
The present study describes novel evaluation modes based on multiphoton microscopy for visualising tissue morphology (e.g., collagen, elastin, and cells) to qualify and quantify the extent of neo-intimal formation of covered arteries and device integration into the arterial wall using a rabbit model of intracranial aneurysms treated with FDS.
Le texte complet de cet article est disponible en PDF.Keywords : Aneurysms, Endovascular treatment, Stenting, Flow-Diverter, Multiphoton microscopy, Second harmonic generation
Abbreviations : FD, HBSS, PMT, SHG
Plan
Vol 48 - N° 3
P. 200-206 - mai 2021 Retour au numéro
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