Point-of-care detection of tuberculosis using magnetoresistive biosensing chip - 11/03/21
, Purva Bhatter b , Vipan Kakkar a Abstract |
In this paper, a highly sensitive and specific technique based on the principle of giant magnetoresistance (GMR) has been proposed for the early stage Tuberculosis (TB) diagnostics. This GMR biosensing assay employs monoclonal antibodies against M. tuberculosis specific ESAT-6 antigen with the use of magnetic nanoparticles (MNPs) as labels. MNPs bind to the GMR sensor in presence of ESAT-6 and the binding is proportional to the ESAT-6 protein concentration leading to the change in overall resistance of GMR sensor. GMR biosensor simulation showed that ESAT-6 concentration can be detected in the range of pg/mL in comparison to the other transduction techniques available for ESAT-6 detection and further, the signal strength increased with the increase in the concentration. This work has shown that the GMR biosensing strategy is pertinent for the TB detection at the primitive phases when compared with other magnetic techniques used for TB diagnostics.
Le texte complet de cet article est disponible en PDF.Highlights |
• | A novel GMR biosensing technique has been proposed for the detection of M. tuberculosis specific protein- ESAT-6. |
• | ESAT-6 is one among the various antigenic proteins secreted by M. tuberculosis at the initial stages of TB infection. |
• | Detection of gene mutations related to drug-resistant TB is possible by DNA analysis using GMR biosensing technique. |
• | Better sensitivity than other magnetic techniques available for TB diagnostics. |
Keywords : Biosensor, Giant magnetoresistance (GMR), Tuberculosis, ESAT-6
Plan
Vol 127
Article 102055- mars 2021 Retour au numéro
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