Metformin decreased myocardial fibrosis and apoptosis in hyperhomocysteinemia -induced cardiac hypertrophy - 12/02/21
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Highlights |
• | Metformin attenuated homocysteine-induced cardiac hypertrophy. |
• | Metformin decreased myocardial fibrosis in homocysteine-induced cardiac hypertrophy. |
• | Metformin attenuated homocysteine-induced apoptosis in vivo and in vitro. |
Abstract |
Background |
Hyperhomocysteinemia (HHcy) is one of the major risk factors of cardiovascular diseases. Metformin acts as a cardioprotective role in several cardiovascular diseases, including ischemia/reperfusion, atherosclerosis, and myocardial infarction. However, whether metformin protects against HHcy-induced cardiac hypertrophy is unclear.
Methods and Results |
HHcy model was established in C57BL/6 mice with high L-methionine (L-MET) diet for 12 weeks. AC16 cells were exposed to homocysteine (Hcy) and then intervened with different concentrations of metformin in in vitro studies. The results showed that HHcy was able to induce cardiac hypertrophy, and metformin could abrogate this effect. HHcy increased the fibrosis area and induced apoptosis in the myocardium, whereas metformin could reverse the detrimental effects above. TUNEL assay showed that metformin was able to decrease Hcy-induced apoptosis in AC16 cells. Moreover, western blotting assay revealed that metformin could decrease Hcy-induced expression of Bax and cleaved caspase3, and increase the expression of Bcl-2.
Conclusions |
This study demonstrates that metformin is able to attenuate HHcy-induced cardiac hypertrophy by decreasing myocardial fibrosis and apoptosis.
Le texte complet de cet article est disponible en PDF.Abbreviations : HHcy, HW/BW, WGA, ANP, TUNEL, Bcl-2, BAX, AMPK, H&E, LVESD, LVEDD, LVEF, FS, L-methionine
Keywords : Homocysteine, Cardiac hypertrophy, Metformin, Apoptosis, Myocardial fibrosis
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Vol 69 - N° 1
Article 103270- janvier 2021 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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