Tanshinone IIA exerts therapeutic effects by acting on endogenous stem cells in rats with liver cirrhosis - 18/11/20
, Haoyuan Chen , Yang Gao , Sijia Zhang , Qiuchi Lin , Xuechun Ji , Ning Li , Wanying Xu , Ying Liu , Shizhu Jin ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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| pages | 10 |
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Graphical abstract |
Highlights |
• | BrdU immunofluorescence double labeling to detect cell proliferation. |
• | Tan promotes the proliferation and differentiation of endogenous hepatic stem cells. |
• | Tan can alleviate liver cirrhosis. |
Abstract |
Background and Objective |
Liver cirrhosis (LC), the major pathway for the progression and development of chronic liver disease, is an advanced stage of liver disease. It is the third most common chronic noncommunicable disease after cardiovascular diseases and malignant tumors. Tanshinone IIA (Tan), an extract of Salvia miltiorrhiza (S. miltiorrhiza), has been proven to promote the proliferation and differentiation of stem cells. Moreover, its protective effect in liver injury has received widespread attention. The present study investigated whether Tan plays a therapeutic role in LC by promoting endogenous stem cell proliferation and differentiation.
Materials and Methods |
LC models were established by intraperitoneal injection of an olive oil solution containing 50 % carbon tetrachloride (CCL4) combined with 10 % alcohol in the drinking water. After successful model establishment, the animals were randomly divided into four groups and injected with physiological saline or low-, medium-, or high-dose (10, 20, or 40 mg/kg) Tan for seven consecutive days. The protective effect of Tan on LC was observed by western blotting, serological examination and histopathological staining. Furthermore, immunofluorescence double-labeling of 5-bromo-2-deoxyuridine (BrdU) and the liver cell markers albumin and CK-18 or the liver stem cell markers EPCAM and OV-6 was used to evaluate the proliferation and differentiation of endogenous liver stem cells.
Results |
We confirmed successful establishment of the LC model by observing transaminase levels and hematoxylin-eosin (HE) and Masson staining of liver sections in CCL4-treated and healthy rats. After Tan treatment, HE and Masson staining of paraffin sections of liver tissue showed that Tan treatment significantly improved histological injury to the liver. Serological tests showed that albumin-bilirubin (ALBI) scores and models for end-stage liver disease (MELD) were lower. Immunofluorescence and immunohistochemical staining showed that the newly proliferated cells were colocalized with ALB, OV-6, EPCAM, and CK-18, indicating that new expression of these markers occurred after Tan injection. All results were most significant in the medium-dose treatment group.
Conclusion |
Tan can alleviate liver injury induced by CCL4 combined with alcohol in rats and plays a therapeutic role in LC by promoting the proliferation and differentiation of endogenous liver stem cells.
Le texte complet de cet article est disponible en PDF.Abbreviations : LC, Tan, ALBI, MELD
Keywords : Liver cirrhosis, Tanshinone IIA, Endogenous stem cells, 5-Bromo-2-deoxyuridine
Plan
Vol 132
Article 110815- décembre 2020 Retour au numéro
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