Annona senegalensis extract demonstrates anticancer properties in N-diethylnitrosamine-induced hepatocellular carcinoma in male Wistar rats - 28/10/20
, Damilohun S. Metibemu b, Isaacson B. Adelani a, Gbolahan O. Adesina a, Chidera B. Edokwe a, Oyinkansola E. Oseha a, Abiodun H. Adebayo a, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 11 |
| Iconographies | 11 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | Hepatocellular Carcinoma (HCC) is the most common primary malignancy while plants are established source of novel drug candidates. |
• | Annona senegalensis demonstrates anti-neoplastic potentials. |
• | Annona senegalensis increased the antioxidant defense system and restored hepatic damages. |
• | Annona senegalensis demonstrate anti-angiogenic, anti-inflammatory and proapoptotic potentials. |
• | Annona senegalensis downregulate the expression of FNTA mRNA involved in Ras’ neoplastic cells proliferation. |
Abstract |
Background |
Hepatocellular carcinoma (HCC) is a common and leading cancer around the globe. This study investigated the anticancer properties of extract of Annona senegalensis in N-diethylnitrosamine (DEN) - induced hepatocellular carcinoma in male Wistar rats.
Methods |
Rats were simultaneously induced with a combination of 100 mg/kg b.wt of DEN and 0.5 mL/kg of carbon tetrachloride (CCl4) intraperitoneally once a week for three weeks in a row. Thereafter, animals were treated with 100 mg/kg and 200 mg/kg b.wt of A. senegalensis extract daily for 21days. Analysis using gas chromatography-mass spectrometry (GC–MS) was carried out to discover the phytoconstituents contained in the n-hexane extract of A. senegelensis. The levels of liver function parameters and antioxidant enzyme activities were determined via spectrophotometric analysis. Reverse transcriptase-polymerase chain reaction technique was used to assess the gene expression patterns of BCL-2, P53, P21, IL-6, FNTA, VEGF, HIF, AFP, XIAP, and EGFR mRNAs.
Results |
Treatment of DEN-induced hepatocellular carcinoma Wistar rats with the extract caused significant (p < 0.05) decrease in the activities of ALT and AST. It also resulted in a reduction of the concentration of MDA and a significant increase (p < 0.05) in SOD and GSH activities. IL-6, BCL-2, VEGF, EGFR, XIAP, FNTA, and P21 mRNAs expressions were significantly (p < 0.05) downregulated after treatment. Histopathological analysis revealed that the extract improved the liver architecture.
Conclusion |
A. senegelensis n-hexane extract demonstrates its anticancer properties by improving the liver architecture, increasing the antioxidant defense systems, downregulating the pro-inflammatory, anti-apoptotic, angiogenic, alpha-fetoprotein and farnesyl transferase mRNAs expression and hitherto up-regulate the expression of tumor suppressor (P21 and P53) mRNAs.
Le texte complet de cet article est disponible en PDF.Keywords : Annona senegalensis, Hepatocellular carcinoma, N-Diethylnitrosamine, Gene expression
Plan
Vol 131
Article 110786- novembre 2020 Retour au numéro
Article précédent
- Enhanced exosome-mediated delivery of black bean phytochemicals (Phaseolus vulgaris L.) for cancer treatment applications
- Javier Donoso-Quezada, Daniel Guajardo-Flores, José González-Valdez
- Extracellular ubiquitin protects cardiomyocytes during ischemia/hypoxia by inhibiting mitochondrial apoptosis pathway through CXCR4
- Yiqun Ji, Jialu Yao, Yang He
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?