Effect of uremic serum on Th17/Treg cell balance and endoplasmic reticulum stress in rats - 28/10/20
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| pages | 8 |
| Iconographies | 5 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | The environmental status of uremic patients is simulated by in vitro experiments. |
• | ERS is one of the imbalance mechanisms of Th17/Treg cells induced by uremic serum. |
• | Sodium citrate can inhibit ERS induced by uremic serum. |
• | The research provides a basis for broadening the clinical application of citrate. |
Abstract |
Background/Aims |
The imbalance of T helper 17 (Th17) and regulatory T (Treg) cells exists in the occurrence and development of various diseases. Endoplasmic reticulum stress (ERS) is an important self-protective cellular response to harmful stimuli, such as uremic environment. The objective of this study was to investigate the Th17/Treg cell balance and ERS in a uremic environment and analyze the relationship between them.
Methods |
(1) The rat spleen lymphocytes were extracted and treated with thapsigargin (inducer of ERS) and sodium citrate. The proportion of Th17 and Treg cells were then detected. (2) The uremic serum-cultured lymphocytes were used and divided into three groups: non-uremic serum group, uremic serum group, and uremic serum + sodium citrate group. Afterward, the proportion of Th17/Treg cells and the expression of ERS-related proteins (GRP78 and CHOP) were detected.
Results |
Thapsigargin had no significant effect on the proportion of Th17 cells within a limited concentration range, but it could reduce the proportion of Treg cells, sodium citrate had a negative influence on the deviation of Th17/Treg cells treated with thapsigargin. Uremic serum treatment reduced the proportion of Treg cells, resulting in an increase of the Th17/Treg ratio. However, sodium citrate had no influence on the deviation of Th17/Treg cells treated by uremic serum. Sodium citrate reduced the elevation of ERS-related proteins induced by uremic serum.
Conclusions |
Uremic serum can lead to the imbalance of Th17/Treg cells as well as ERS, suggesting that ERS is one of the mechanisms of the imbalance of Th17/Treg cells induced by uremic serum. Sodium citrate can inhibit ERS induced by uremic serum.
Le texte complet de cet article est disponible en PDF.Keywords : Th17 cell, Treg cell, Endoplasmic reticulum stress, Uremia
Plan
Vol 131
Article 110705- novembre 2020 Retour au numéro
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