Structural characterization, hypoglycemic effects and antidiabetic mechanism of a novel polysaccharides from Polygonatum kingianum Coll. et Hemsl - 28/10/20
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Graphical abstract |
Highlights |
• | A novel homogeneous polysaccharide (PKPs-1) was isolated from Polygonatum kingianum. |
• | The physicochemical characterization of PKPs-1 was analyzed. |
• | PKPs-1 shows high hypoglycemic properties in diabetic mice, and the underlying mechanisms were elucidated. |
Abstract |
The rhizome of Polygonatum kingianum has been used as a traditional medicine in China. In this study, a novel polysaccharides (PKPs-1) was isolated from P. kingianum and characterized by its molecular weight, primary structure. The hypoglycemic activity of PKPs-1was investigated by in vitro assay with the HepG2 cell line and in vivo test using STZ-induced diabetic mice. Results showed that the average molecular weight of PKPs-1 was 14.05 kDa and is composed mainly of glucose and mannose. Methylation analysis indicated that this polysaccharides fraction consisted mainly of β1,2-link glucose. Besides, PKPs-1 exhibited significant anti-hyperglycemic activity on STZ-induced mice, improved insulin tolerance, and affected the metabolism of serum lipids. Results of real-time quantitative PCR (RT-PCR) showed that PKPs-1 significantly increased the expression of insulin receptor substrate-1 (IRS-1), phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT), indicating that PKPs-1 regulates glucose metabolism by activating the PI3K/AKT signaling pathway. This study provides new insights for investigating the hypoglycemic effects of PKPs-1 and suggests that PKPs-1 could be a promising functional food or medicine for treating T2DM.
Le texte complet de cet article est disponible en PDF.Keywords : Polygonatum kingianum, Polysaccharides, Structural characterization, Hypoglycemic effects, Type 2 diabetes
Plan
Vol 131
Article 110687- novembre 2020 Retour au numéro
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