Exosome secreted by human gingival fibroblasts in radiation therapy inhibits osteogenic differentiation of bone mesenchymal stem cells by transferring miR-23a - 28/10/20
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Graphical abstract |
Highlights |
• | Irradiation activated gingival fibroblasts inhibit osteogenic differentiation of hBMSCs. |
• | miR-23a/CXCL12 regulate osteogenic differentiation of hBMSCs. |
• | Exosomal miR-23a released from irradiation activated gingival fibroblasts. |
Abstract |
Radiation-induced fibrosis is recently established as a main reason for osteoradionecrosis of the jaw (ORNJ), anti-eradiation fibrosis drugs achieve satisfactory therapeutic effects. However, the molecular mechanism remain to be fully elucidated. In this study, we found the inhibitory effect of irradiation activated gingival fibroblasts on osteogenic differentiation of human bone mesenchymal stem cells (hBMSCs). Moreover, irradiation-activated-fibroblasts significantly increased miR‑23a expression in hBMSCs. Decreased miR‑23a enhanced osteogenic differentiation of BMSCs, and elevated miR‑23a inhibited this process via directly targeting CXCL12. Finally, exosome released from irradiation-activated-fibroblasts inhibited osteogenic differentiation of BMSCs, and these exosome mediated delivery of miR-23a and further regulated miR-23a/CXCL12 axis in hBMSCs. Therefore, our findings suggest that by transferring miR-23a, exosome secreted by human gingival fibroblasts in radiation therapy serves a vital role in osteogenic differentiation of hBMSCs, which may provide novel clinical treatments for ORNJ.
Le texte complet de cet article est disponible en PDF.Abbreviations : ORNJ, RI, BMSCs, CXCL12, RT-qPCR, RIF
Keywords : Exosome, Irradiation therapy, Myofibroblasts, Bone marrow-derived mesenchymal stem cells, miR-23a
Plan
Vol 131
Article 110672- novembre 2020 Retour au numéro
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